| Objective:Platelet-rich fibrin (PRF) is a new generation of blood concentrates, rich in a variety of growth factors. In this study, we establish the goats mandibular defect model, taking oral collagen membranes and no treatments as control groups, to explore the PRF in the role of bone regeneration in order to provide a reference for its clinical research and application.Methods:Three 8-12 month healthy male goats were used for this study and three round shaped defects(diameter 5mm,2mm deep) were formed in the buccal ridge of the bilateral mandibular anterior edentulous area by using the method of self control. The three defects were divided into three groups (Group A,Group B and Group C). Group A received PRF membranes as the experimental group; Group B received Haiao collagen membranes as the standard control group; Group C did not received any grafts as the blank control group. Animals were killed at 4,8 and 12 weeks after operation. The specimens were examined by gross observation,X-ray,HE histologic observation to compared the osteogenic effect. HPIAS-2000H high-resolution colour pathological graph-text analytical system was used to calculate the percentage of new formed bone area. SPSS 13.0 for statistical analysis.Results:1. Gross observation:In this study, there were no animal deaths,having a good surgical healing, no wound infections and the colour of oft tissue were normal. At 4 weeks, the blank control group was not completely filled, the experimental group and standard control group was almost healed. At 8 weeks and 12 weeks, all the defects were filled with bone, there were no significant difference between the three groups.2. X-ray observation:At 4 weeks,it was showed clear small circular radio-transparent spot in the blank control group,while there were smaller radio- transparent spots with unclear border in the experimental group and standard control group.At 8 weeks, the radiotransparent spots were almost invisible in the experimental group and standard control group while it was still a little visible in the blank control group.At 12 weeks there were no significant difference in the three groups.3. HE histologic observation:At 4 weeks,there were less new bone formation, fewer osteoblasts,more fibers in the blank control group than those in the experimental group and standard control group.At 8 weeks, in the experimental group and standard control group, the new formed bone thickened and connected into networks,the quantities of osteoblasts were encreased and the fibrous tissue decreased.While in the blank control group there is relatively thin trabeculae, more fibrous tissue.At 12 weeks, the experimental group and standard control group showed visible lamellar structure and thicker trabeculae,while the blank control group showed no lamellar structure.4. Bone histomorphometry analysis:the percentage of new bone area at 4 weeks, 8 weeks and 12 weeks in the experimental group (35.92±3.89,60.72±4.53, 74.73±3.64 respectively)were higher than those in the blanck control group(22.56±4.59,52.18±5.31,66.77±4.52 respectively), the difference was statistically significant (P＜0.05). The percentage of new bone area at 4 weeks,8 weeks and 12 weeks in the experimental group were not significantly different from those in the standard control group (34.36±3.61,61.95±5.24,76.24±3.35 respectively)(P> 0.05).Conclusions:1. Platelet-rich fibrin plays a significant role in promoting bone regeneration of bone defect area.2. Platelet-rich fibrin can be applied alone as graft for bone defects when the defect is relatively small. |
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