The Study Of Puerarin Sold Self-microemulsion Drug Delivery System

OBJECTIVE In order to improve the bioavailability of puerarin, we made it into puerarin ordinary self-microemulsifying drug delivery system(SMEDDS), and determine the prescription of puerarin SMEDDS.On the basis of puerarin SMEDDS, we aad Panax Notoginseng Saponins (PNS) into the puerarin SMEDDS in order to reduce the amount of emulsifier to achieve the purposes of synergy and attenuation.METHODS The solubility of puerarin in different surfactant was investingated. The basic prescription was determined by pseudo-ternary phase diagram obtained by titration method. On that basis, adding an appropriate proportion of PNS,selecting the self-emulsifying efficiency and the emulsion particle size as the index to optimize the SMEDDS formulation anddetermine the final formulation. HPLC(high-performance liquid chromatography)method was adapted to determine the dissolution and the in vivo pharmacokinetics of puerarin S-SMEDDS of rats.RESULTS The final formulation was Ethyl oleate:Tween-80/EL-40 (1:2): PEG: puerarin: PNS = 15:35:35:6:9.The solid absorbent material is Mannitol, the average diameter was 32.9nm and the relative bioavailability is 238.77%.CONCLUSION The acquired microemulsion with PNS has small particle size and good stability, which provides a new way of thinking and approach for SMEDDS to reduce toxicity and increase efficacy.