| Background and objectiveEsophageal cancer is a common malignant tumor, its incidence and mortality is in the fourth place in our country. Due to early diagnosis is not easy, about 70 percent to 80 percent of patients have entered the clinical middle-late stage when they come to the hospital. Surgery has been regarded as the standard of esophageal cancer treatments, but even if it is relatively early postoperative pathological changes, the recurrence rate is still high. At present,the survival rate of esophageal cancer overall five years is about 30%, curative effect has to be improved. In recent years, with the rapid development of clinical oncology, the necessity of perfect esophageal synthesis therapy and rapid development becomes more and more serious, has attracted the attention of clinical research and has come to a hot topic. Medical treatment of esophageal cancer is still stay in cytotoxic drugs level. So looking for new molecular targets, discovering new targeted therapy drugs has become vitally significant.Tissue factor pathway inhibitor, TFPI-2,is also be called placental protein, PP-5, is an analogues of TFPI, is a family member of Kunitz type Serine trypsin inhibitors. It has inhibited effection to various enzymes, including matrix metalloproteinases, trypsin, fibrinolytic enzyme and so on. MMPs participated in the remodeling activityof extracellular matrix, at the same time it plays an important role in the physiological and pathological processes,such as fibrinolysis, atherosclerosis, wound healing and tumor cells infiltrating transfer and angiogenesis. Recently, TFPI-2 gene is considered as a new candidate tumor-suppressor gene, more and more studies show that TFPI-2 significantly suppressed the function of MMPs in the activity of tumors, it can control the invasion and metastasis of malignant tumor cells, according to maintaining the integrity of the extracellular matrix tumor, regulating cell apoptosis and angiogenesis tumors. The exact role of promoter methylation CpG island high area in tumor formation mechanism is unclear. However, many evidences indicated that, abnormal methylation of tumor suppressor CpG island, causing gene inactivation and transcription repression, is one of the important mechanism of the cancer. The CpG island had methylation is reversible, through artificial or other factors intervention, it can make CpG island of relevant genes to be methylation and restore its expression. As a result, the methylation treatment may be expressing is a new idea to the treatment of negative TFPI-2 of esophageal cancer.At present, the analysis of TFPI-2 gene expression in esophageal cancer is less, the object of this study is to explore the cell proliferation and growth of esophageal squamous cell cancer after the intervention of 5-Aza-2-Deoxycytidine (5-Aza-CdR), and the influence on the gene expression of plant tissue factor pathway inhibitor 2 (TFPI-2).MethodsEC9706 cell line was treated by 5-Aza-CdR of different concentration, MTT, flow cytometer, Immunohis-tochemistry and RT-PCR were used to determine the cell growth, apoptosis, the expression of TFPI-2 gene and its protein.ResultsAfter 5-Aza-CdR treated, the proliferation of EC9706 is inhibited, the apoptotic rate was also increased significantly in a concentration dependent manner, RT-PCR detected mRNA of TFPI-2 gene in EC9706 cell line recovered express.Conclusion(1) The silence of tumor-suppressor gene TFPI-2 expression is related to the occurrence of esophageal cancer.(2) 5-Aza-CdR can restrain the proliferation of esophageal squamous cell carcinoma EC9706 cell, and promote the cell apoptosis.(3) 5-Aza-CdR may inhibit esophageal cancer cell proliferation, and promote apoptosis,by eliminating the methylation state of tumor-suppressor gene TFPI-2, making its new expression. |
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