| Background The greatest theat to health of diabetes was complications. One of the complications was diabetes with refractory wound, which was a challenge facing the Medical. Its pathogenesis was complex, and didn't have effective treatment. How to repair the refractory wound in diabetes was an important unresolved problem of clinical and research. In recent study, transforming growth factor-β1 was an important factor involved in the process of wound healing, and its abnormal functions and numbers due to the refractory wound.Objective To observe the expression of Transforming growth factorβ1(TGF-β1) in the tissues of second-degree scald in diabetic rats, and the effects of exogenous TGF-β1 on wound healing process of second-degree scald in diabetic rats, approach the molecular biology mechanism and find new fundament for the therapy of scald in diabetic rats,Methods Eighteen adult male SD rats(±230g) were randomly divided into 3 groups: scald group (control group), diabetic scald group (diabetic group) and diabetic scald with TGF-β1 treatment group (TGF-β1 group) (n=6). Rats in diabetic group and TGF-β1 group were treated with intraperitoneal injection of 55mg/kg streptozotocin to induce diabetes. Body weight were observed and blood glucose levels were monitored from samples obtained by tail blooding at 24 hours,7, 14days after injection to set diabetic model. Then we settled deepⅡdegree scalded model 1 month after diabetic model establishment.24 hours before burning, all rats were anesthetized with 10% hydral, and epilated of back hair.24 hours after epilation, all rats were made to deepⅡdegree scalded injury on the back by 100℃counterweight with 2cm diameter (control group: 13s; diabetic group, TGF-β1 group:11s), and immediately followed by intraperitoneal injection of 5ml normal saline(NS) to prevent shock. After model establishment, local intradermal injection of 0.5ml TGF-β1 (5ng/ml) in the wound was performed in TGF-β1 group every 2 days. The unhealed areas of wound were recorded and wound healing rates were calculated 1,3,5,7,14 and 21 days after scald. Healing process was evaluated by histological examination, and by taking the tissues of wound margin, expression of TGF-β1 was detected by immunohistochemiscal technique. Dates were analyzed statistically. SPSS 13.0 statistical package was used to deal with all the data. There was significant difference when P<0.05.Results By observation of wound healing and histological examination, wound healing was more hysteretic in diabetic group than that in control one at each time point. The first three days after scald, there was no significant difference between each groups(all P>0.05), Five days after scald, the healing rate in control group and TGF-β1 group was significantly higher than that in diabetic group (P<0.05), while there was no significant difference between control group and TGF-β1 group. Compared with control group, the expression of TGF-β1 was lower in the tissues of scald in diabetic group (P<0.05).Conclusion The unfavorable healing process is related to decreased amount of TGF-β1 in diabetic wound. Topical application of TGF-β1 can accelerate the wound healing in diabetic rats. |
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