The Research On The Application Of Fluorescence In Situ Hybridization In Diagnosis Of Urothelial Cancer

Objective:To compare the application of fluorescence in situ hybridization(FISH) and urine exfoliocytology in diagnosis of urothelial cancer.Methods:Twenty specimens were collected from healthy persons. The abnormality of the third,7th,17th chromosome and p16 dot at 9th chromosome were detected with FISH technology in order to calculate the corresponding threshold.89 patients with hematuria, who were treated in minimally invasive urology center of Shandong Provincial Hosipital from June 2010 to March 2011, were chosen. Fresh urine samples from them were collected and detected with FISH technology and urine exfoliocytology. The sensitivity and specificity of the two tests were analyzed and compared.Results:Of the 89 patients,67 had been proven to be urothelial cancer by histology. The total sensitivity of urine exfoliocytology and FISH were 52.2% and 89.6%(P=0.000), and their specificity was 95.5% and 90.9% (P=0.546), with the diagnostic concordance rate of 62.9% and 89.9% (P=0.000), respectively; their sensitivity of non muscle-invasive cancer was 26.5% and 75.5% (P=0.000), respectively; their sensitivity of muscle-invasive cancer was 78.8% and 94.4% (P=0.136), respectively. The difference between FISH and exfoliocytology showed significance in the total sensitivity, diagnostic concordance rate and the sensitivity of non muscle-invasive cancer, with no significance in the specificity and the sensitivity of muscle-invasive cancer.Conclusions:According to these results, FISH has a higher sensitivity than urine exfoliocytology and a similar specificity in detecting urothelial cancer, and could be a new and excellent detecting technology for the early diagnosis and prodicting the prognosis of urothelial cancer.