Preparation And Quality Evaluation Of Cinnamon Oil Microemulsion

In this research, the microemulsion of cinnamon oil was prepared by the method of pHase inversion emulsification. The quality stability and physicochemical property of cinnamon oil microemulsion were studied, the analytical method of cinnamicaldehyde in plasma were established. Investigate delivering situations of Cinnamon oil microemulsion in vitro. The main results were as follows:1. Preparation of cinnamon oil microemulsion.The thesis investigates the interaction among oils, surfactants and co-surfactants. According to the size of the microemulsion as the index, the optimum formulation was investigated by using titration to prepare the Pseudo-ternary phase diagram, testing the size of distribution and physicochemical property of cinnamon oil microemulsion. The results showed that the cinnamon oil microemulsion delivery could be prepared using EL-40/Tween80/dehyrated ethanol/IPP/cinnamon oil/Water(Km=2,EL-40/Tween80=2).The average diameter of cinnamon oil microemulsion was 32.0 nm, Its basic character parameter such as refractive indexes, pH, conductivity, viscosity were 1.415nd,5.24, 111us/cm,105mpa.s,respectively. The centrifugal acceleration experiment certify that microemulsion have a good stability.2. Established the HPLC method for determining the content of cinnamicaldehyde in cinnamon oil microemulsion.The calibration curve was linear in the range of 28.8～100.8μg/mL (R2= 0.9999). The mean recovery was 101.29% and RSD was 1.45%. The formulation is reasonable and the method is accurate.3. The stability quality evaluation of cinnamon oil microemulsionResearch stability of cinnamon oil microemulsion was at 4℃, room temperature, 30℃and under light. At the temperature of 4℃, cinnamon oil microemulsion is white solid, it turns to be clear, transparent, uniform system when heat it. The content of cinnamicaldehyde is decrease slowly. The content of cinnamicaldehyde averagely declined 25.2%. At the temperature of 30℃, the appearance of cinnamon oil microemulsion was kept well between zero and the fifth months, there are a few of trace suspended solids at the sixth month. The content of cinnamicaldehyde averagely declined 60.5%. At the room temperature, the appearance of cinnamon oil microemulsion was kept well between 0 and the fifth month, there are a few of trace suspended solids at the sixth month.The content of cinnamicaldehyde averagely declined 53.6%. Illumination experimental results, the appearance of cinnamon oil microemulsion was kept well at the tenth day, The content of cinnamicaldehyde averagely declined 16.4%. These results suggested that cinnamon oil microemulsion should be store at low temperature and away from light.4. Established the HPLC method for determining the content of cinnamicaldehyde in sheep plasmaMethanol was usd to precipitate the plasma protein. At the time of cinnamicaldehyde's chromatogram peaks, there are no other chromatogram peaks in the blank plasma's chromatogram. The experimental specificity is good. The calibration curve was linear in the range of 216μg/mL～4500μg/mL (R2= 0.9995). Extraction recovery and methods recovery are meeting the requirement of experiment.5. Investigated the delivering situations of Cinnamon oil microemulsion in vitroPreparation artificial intestinal liquid (6.8 pH) for the research. Investigate delivering situations of Cinnamon oil microemulsion in vitro. Put the samples in table concentrator,controlling temperature in 37±0.5℃, speed at100r/min.Taking 4mL samples respectively (0.5 hours,1 hours,2 hours,4 hours,6 hours and 8 hours) to investigate the content of cinnamaldehyde. The results showed that the release of cinnamon oil microemulsion in vitro matched with Retger-perpas equation model. (R2=0.93978)...