Studeis On Protective Effects Of Gypenosides On PC12 Cells Injured By Oxidative Stress

Ischemic cerebrovascular disease (ICD) is the central nervous system disease caused by the artery block temporary or permanent. It approximately holds 80%in the cerebrovascular disorders. Although the treatment of the ICD has gained some expectativelly progress in the animal model but was disappointed in clinic experiment. Currently thrombolysis is the main therapy method, but there are some dangers and restrictions for hemorrhage and reperfussion injury. Furthermore the occurrence of reperfussion is very frequently in ischemic brain tissue and therefore its damage will inevitable.Cerebral ischemia-reperfusion(CIR)injury is the damage even severely when the blood stream recanalize in the ischemic brain tissue which has experienced cerebral ischemia for some time. Therefore investagate the mechanism of the cerebral ischemia injury and actively develop the protective drugs for the damage have very important theoretical significance and utility value.Gypenosides (GP) is a traditional Chinese herbal medicine. The effect of GP on cerebral ischemia has few been reported in China at present. Domestic research materials showed that GP has the effect of improving arterial blood streaming and ameliorating the blood viscosity, anti-thrombotic effect, inhibiting platelet aggregation. But we don't know wether GP has protective effect on cerebral ischemia. Its mechanism need continue to be investigated.We used 0.1mmol·L-1 hydrogen peroxide (H2O2) to damage pheochromocytoma cell (PC 12 cell). PC 12 cell is an ideal model because it can be easily cultivated and has basal neuron biological characteristics. H2O2 is one of middle metabolizing products of the oxidative metabolism. Its accumulation in the body may lead to toxic effect on cells. The survival ability was detected by methyl thiazolyl tetrazolium (MTT) method. The contents of LDH, SOD and MDA in cultured conditions were measured by using the instruments. The cell apoptosis rate was detected by flowcytometry. Protein of Bcl-2 and Bax were detected. The results showed that GP could increase the survival rate of PC 12 cells, reduce the release of LDH from PC 12 cells, increase the activity of SOD, reduce the concentration of MDA and PC 12 cells apoptosis percentage. At the same time, GP could upregulate the protein expression of Bcl-2 and inhibit the expression of Bax. It is suggested that GP had protective effect through promoting the protein expression of Bcl-2 and inhibiting the expression of Bax and inhibiting neurocyte apoptosis possibly.From the above, we can make a conclusion that protective effects of GP on oxidative stress damaged by H2O2 of PC 12 cells are probably through strengthening anti-oxidative enzyme activity, improving the ability of cleaning free radicals, inhibiting lipid peroxidation injury, upregulating the expression of Bcl-2, inhibitting the expression Bax and inhibiting neuron apoptosis.