Relationship Between The Intestinal Mucosal Barrier Damage And Alteration Of Toll-like Receptor 2 In Rats Intestinal Ischemia/Reperfusion Injury

Objective:To investigate the relationship between the Intestinal Mucosal Barrier Damage and Alteration of Toll-like receptor 2 in rats, and to study the association of the mechanism of inflammation damage in small intestine and SIRS or MODS after IIR with native immunity be mediated by TLR2. Methods:The murine model of intestinal I/R was constructed by clamping the superior mesenteric artery.60 Wistar rats were randomly divided into 10 groups as follows:normal control group (group A,), sham operation group (group B), group C to E with intestinal ischemic duration of 30,40,50 min and group F to J with reflow after 2-hour,6-hour,12-hour,24-hour and 48-hour, respectively (6 for each group).The expressions of Toll-like receptor (TLR) 2 and DAO were detected by Enzyme-linked immunosorbent assay (ELISA), and taking part of small intestine to make compare with HE staining. Results:There is a significant change after intestinal tisuue in HE staining, and the levels of TLR2 in the serum, small intestine, liver, peyer's petch nodes and DAO in the serum increased dramatically(p<0.05) in IIR model group, compared with normal control group, by ELISA. TLR2 invarious tissues and serum was positively correlated with sampling time. Peyer's petch TLR2 expression in lymph nodes, and compared with the control group was statistically significant (p<0.05), but in other organizations, the TLR2 responsed more slowly. Conclusion:TLR2 increased expression in a variety of tissues by intestinal ischemia-reperfusion injury. And the alteration of TLR2 was relevant to Intestinal infection, development and destruction of intestinal mucosa barrier. This result to may be happened by natural immune response and postnatal immunity, which be caused by signaling pathway of TLR2 inflammatory mediators in intestinal mucosa were activated.