The Research About The Affect Of Ulinastatin On The Expression Of HMGB1in Intestinal Mucosal And Intestinal Mucosal Barrier In SAP Rats

Objective: To explore the affect of Ulinastatin for severe acute pancreatitis (severe acutepancreatitis, SAP) rat intestinal mucosa high mobility group protein B1(high mobilitygroup box-1protein, HMGB1) expression and role of mucosal barrier, provideexperimental basis for clinical intervention in severe acute pancreatitis.Methods: Experimental animals chosen female SD rats of clean grade,weighing220-250g,the average weight of237.45g, divided into control set,SAP set and ulinastatinintervention set at random,n=18,all rats were fasted for12hours before theexperiment,without limiting the water, punctured duodenal and retrogradecholangiopancreatography injected5%sodium taurocholate to induce SAP rat model, incontrol set, pancreas was just flipped.Five minutes after the SAP pattern successfullyinduced,injected ulinastatin(1000IU/100g) in the tail vein within ten minutes,the controlset and SAP set were injected normal saline.Each set was divided into three subsetsaccording to6h,12h,24h after modeled,each subset of6.According to the three point intime,the rats were killed in three batches,each batch of six. Automatic biochemical analzyer was used to detect the levels of serum amylase,Enzyme-Linked ImmunosorbentAssay (ELISA) was used to detect intestinal HMGB1and serum TNF-α levels,Spectrophotometric Determination was used to detect intestinal enzyme diamine oxidasecontent, observed the histological changes of the pancreas,and measured the wet and dryratio of intestinal tissue..Results: Pancreas swelled significantly and a bit like bled when successfullymodeled[1].10rats of SAP set,6rats of ulinastatin intervention set and3rats of control setdied,then re-modeled to supplement date.Pairwise comparison had been used in serumamylase levels, serum TNF-αlevels, intestinal HMGB1levels, intestinal diamine oxidasecontent, wet and dry ratio of intestinal tissue, pancreatic tissue pathology score in controlset, SAP set and intervention set of6h,the difference was statistically significant(p<0.05).The difference was also statistically significant during these indicators in controlset, SAP set and intervention set of12h(p<0.05).The difference about serum TNF-αlevels in SAP set and intervention set of24h was not statistically significant(p>0.05),butcompared with the control group, the difference was statistically significant(p<0.05). Thedifference of rest indicators in control set, SAP set and intervention set of24h wasstatistically significant(p<0.05).Conclusion: we can successfully create an animal model of SAP by the method.Ulinastatin can reduce the expression of inflammatory cytokine HMGB1in intestinaltissue.Ulinastatin can protect intestinal cells, reduce the loss of diamine oxidase in theintestinal mucosa, reduce wet and dry ratio of intestinal tissue, protect the intestinalmucosal barrier function.