Effect Of Heat Shock Protein 70 Induced By Alanyl Glutamine On Inhibiting Tubulointerstitial Fibrosis In Unilateral Ureteral Obstruction Rats

Objective To investigate the effect of heat shock protein 70 induced by alanyl-glutamine on inhibiting tubulointerstitial fibrosis in unilateral ureteral obstruction rats.Method 20 male wistar rats weighing 200~250g were randomly divided into four groups:sham group,UUO model group,GLN group and QUE group. Rats subjected to UUO operation were treated with daily injection dose of 1.125g/kg Ala-Gln or 0.9% NS one day before UUO operation until being sacrificed.Rats of QUE group were treated with daily injection dose of 400mg/kg quercetin one day before injection of Ala-Gln. Five rats were sacrificed at day 7 in each group.Routine kidney pathology and Masson assay was examined.Expression of HSP70,NF-κB and a-SMA protein was assessed by immunofluorescence and Western-blot.Results GLN group markedly elevated HSP70 level in kidneys.As compared to UUO model group and QUE group,GLN group significantly reduced tubular lesions and attenuated interstitial fibrosis [ ( 26.2%±1.6% vs 78.4%±3.5%,80.2%±3.3%),score(1.32±0.08 vs3.67±0.22,3.70±0.20),P <0.05]; TEMT was significantly reduced by down-regulation of NF-κB and a-SMA protein (P<0.05)Conclusion Ala-Gln can markedly attenuate tubulointerstitial fibrosis in UUO rat model by up-regulation of HSP70 in kidneys ,and the inhibition of TEMT and a-SMA by down-regulation of NF-κB is possibly one of the potential mechanisms.