In Vitro Amplification Of CD4~+ CD25~+ Regulatory T Cells

Objective:To explore separation and amplification method of CD4+CD25+T cells in vitro.Methods:By micro-magnetic beads method (MACS) isolating CD4+CD25+T cells; By using IL-2 (200IU/mL)+anti-CD3/CD28/beads to expansion in vitro after the separation of CD4+CD25+T cells.Results:1. Using MACS separate adult peripheral CD4+CD25+T cells,the purity of the cells up to 81-91%.2. The expansion methods of using IL-2 (200IU/mL) +anti-CD3/CD28/beads can reach a certain degree of amplification effect.Conclusion:Using MACS separation adult peripheral CD4+CD25+T cells is feasible, separation of high purity. Adopt IL-2+anti-CD3/CD28/beads method amplification CD4+CD25+T cells can reach significant amplification effect.