| Objective To analyze the expressions of delta-opioid receptor(DOR),β-arrestin1 and Bcl-2 in rats with experimental ulcerative colitis(UC) will help to studying the effects of DOR-β-arrestin1-Bcl-2 signaling on the immunologic pathogenesis of ulcerative colitis and the effects of Wumeiwan on the therapeutic mechanism of ulcerative colitis. Methods Fifty-six SD rats were divided into model group, Wumeiwan group,mesalazine group and control group with 14 each randomly. All the experimental rats without control group were treated with 2, 4, 6-trinitrobenzenesulfonic acid to induced ulcerative colitis. The rats of control and model groups were given 3mL normal saline, then the Wumeiwan(0.51g/L) and the mesalazine solution(0.5g/L) as the treated teams, the experimental rats were given 3mL solution everyday for fifteen days. The expressions of DOR,β-arrestin1 and Bcl-2 of colonic tissues were determined by immunohistochemistry and real-time quantitative polymerase chain reaction (RT-PCR).Results The expressions of DOR,β-arrestin1 and Bcl-2 were significantly different among the four groups(P <0.05). The expressions of DOR,β-arrestin1 and Bcl-2 protein and mRNA were significantly increased in model group compared with the other groups(P <0.05). However, the expressions of DOR,β-arrestin1 and Bcl-2 were significantly decreased after treating with mesalazine and Wumeiwan (P<0.05), and there is no statistically differences between mesalazine and Wumeiwan treatment group (P >0.05).Conclusion DOR-β-arrestin1- Bcl-2 signal transduction might play a central role in perpetuation of chronic experimental UC. Inhibiting the expressions of DOR,β-arrestin1 and Bcl-2 and intervening DOR-β-arrestin1-Bcl-2 signal transduction is one of mechanisms of WuMeiWan attenuating ulcerative colitis. |
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