The Effect Of HBV Infection To The Expressionof KU80 In Human Hepatocellular Carcinoma

Objective HBV infection and the incidence of human hepatocellular carcinoma(HCC) are closely related,and the key element KU80 in DNA double-strand damage repair plays an important role in the development of HCC. But the expression of KU80 after HBV infection in HCC is unkown, and currently no studies reported the exact. We will study the effect of HBV infection to the expression of KU80 in HCC.Methods We collected 40 cases of surgical specimens of HCC, including 10 HBV-negative patients and 30 HBV-positive patients. Immunohistochemistry and Western blot were used to detect the expression of KU80 protein in HCC and paracancerous tissues from HBV-negative patients and HBV-positive patients. The expression of KU80 protein in human L02 cells treated with serum HBV,human L02 cells transfected with pcDNA3.1-HBX and human L02 cells transfected with empty plasmid was measured by Western blot.Results About 75%(30/40)cases, the expression level of KU80 in HCC was significantly lower than its paracancerous tissue. After further study by groups, we found the rate of reduce of KU80 expression in HBV-positive patients was higher than HBV-negative patients(26/30vs4/10), and has statistically significant difference(P<0.05). As the treatment time extended, the expression level of KU80 in human L02 cells treated with serum HBV has obvious trend of reduction, and compared with human L02 cells transfected with empty plasmid, the expression level of KU80 was significantly reduced in human L02 cells transfected with pcDNA3.1-HBX.Conclusions Compared with paracancerous tissue, the expression level of KU80 was significantly reduced or missing in HCC. Maybe as a result from HBV infection or HBX gene integration, the expression level of KU80 was reduced in HCC.