Study On Magnetic Field's Effect And Dose-Effect Relationship On Fibroblast

With the recent rise in the popularity of noninvasive drugless therapy and the increasing emphasis on healthcare maintenance, magnetic treatments are getting more and more attention because it is simple to use and painless. Its development and biological effects are now often reported in the media.The research on magnetic therapy is at its infancy and the clinical use is still not strictly regulated. Its dose-effect relationships, effect on the biological internal mechanism and overall safety are unclear. Therefore much theoretical work, systematic research, and in-depth explorations are still necessary. From applying spatial magnetic field from permanent magnet for magnetotherapy, this study analyzed the effect and dose-effect relationship on fibroblast and organic mechanisms under both normal and hypoxic conditions.An outline of the main research follows:1. Analyzed the spatial distribution of magnetic induction of the round permanent magnet and designed methods to apply to in vitro cell culture accurately for carefully designed levels of magnetic strength.2. Measured cell activities by MTT to study the effect of magnetic field on fibroblast proliferation. Results showed that selected magnetic fields with varying magnetic induction strengths all increased the fibroblast activities to different degrees.3. By measuring LDH in the cell culture medium, studied the effect of magnetic fields on fibroblast cell membrane damage. It was found that most of the selected magnetic fields in the experiment do not damage fibroblast cell membranes.4. By measuring SOD and MDA in cell culture medium, studied the effect of different magnetic induction on cells from oxidative injury. The result was that cell antioxidant was enhanced, but the oxidative injuries varied.5. Using flow cytometry detect the effect of magnetic field on fibroblast apoptosis. The results identified the effect from different levels of magnetic field strength and the associated safety thresholds. But the study could not extrapolate a simple rule between the magnetic induction strengths and the apoptosis results. This paper researched the effect from applying magnetotherapy on fibroblast, measured its effects from well-designed strength levels and amounts, and provided basic experimental evidence from using a standard permanent magnetic field. Effective magnetic strengths, dosages, and associated safety thresholds were accurately documented.