Preparation And Character In Vitro Release Of PLA Nanoparticles Incorporating INH And RFP

Objective To study characteristics of PLA sustained release nanoparticle(nps) incorporating Isoniazid(INH) and Rifampicin(RFP) through release in vitro,in order to provide new targeted carrier of anti-tuberculosis chemotherapy.Methods⑴INH-RFP-PLA-NPS using modified spontaneous emulsification solvent diffusion method and stored by freeze-dried.⑵The submicron particle size analyzer was used to determine the nanoparticle size and distribution,observing the morphology of nanoparticle with Transmission Electron Microscope.⑶High Performance Liquid Chromatography(HPLC) was used to construct method of determination and determine drug loading(DL) and entrapment efficiency(EE) .⑷Phosphate buffer solution(PBS) was used as a medium to study the characteristics of release in vitro of INH-RFP-PLA-NPS.Results⑴The morphology and nanoparticle of INH-RFP-PLA-NPS were smooth and uniform ,no obvious adhesive phenomena.⑵The submicron particle size analyzer was used to determine nanoparticle size and distribution, the mean diameter was 80.4nm and distribution uniform.⑶The drug loading and entrapment efficiency of INH were 15.95±1.34(%)and 5.01±0.17(%), RFP were 4.66±0.97(%) and 4.05±0.18(%),respectively.⑷Release in vitro,the result of INH-RFP-PLA-NPS was good,the rate of INH was 15.22% in burst release phrase, and rose to 95.6% after 3 days, RFP was 9.26% and rose to 90.3% after 3 days.Conclusions⑴INH-RFP-PLA-NPS using modified spontaneous binary solvent diffusion method, the mean diameter were small and uniform.⑵Release in vitro of INH-RFP-PLA-NPS was stable, no obvious burst release phenomena.