Study On The Expression Of PGC-1α In Multiple Myeloma And Myeloma Related Angiogenesis

Objective:The PGC-1αis one of the most important coactivators regulating gene transcriptional activation. One study proceeded by Arany indicated that this transcriptional coactivator can promote angiogenesis. The study aimed to describe the expression of PGC-1αin multiple myeloma and to explore the relationship between its expression and microvessel density.Methods:The protein level expression of PGC-lα/βwas tested by immunohistochemistry. RT-PCR was used to exam some regulating factors involved PGC-la pathway. We apply Anti-CD34 to quantify the MVD in bone marrow biopsy.Results:Our experiments first certified high protein expression level of PGC-1αin multiple myeloma. Amazingly, we find that PGC-1αwas merely high expressed in MM among all tested malignant hematologic specimens. In the same biopsy, the expression level was parallel with bone marrow microvessel density. PGC-1β, another PGC-1 family member, was discovered in different diseases. The expressed protein of PGC-1αin myeloma biopsy was located at cytoplasm, but in myeloma cell line RPMI8226 and U226 nuclear instead. And the results by RT-PCR revealed that some factors involved PGC-1αpathway were also expressed in bone marrow biopsy, such as MEF2C and ERRa.Conclusions:High expressed PGC-1αand related modulating factors in myeloma may play a key role in angiogenesis generating in bone marrow microenvironment.