Inhibition Of LRIG1 Gene In The Transfected GL15 Cell Line Affects The Expression Of P-EGFR, P-AKT And P-ERK

Objective Inhibition of LRIG1 gene affects the levels of p-EGFR, p-Akt and p-ERK protein of the transfected GL15 cell line.Methods The transfected GL15 cell were cultured in medium containing G418. After EGF (100ng/ml) stimulated the cell, the levels of p-EGFR, p-Akt and p-ERK protein were detected with Western Blotting.Results The levels of p-EGFR and p-Akt protein rised after 5 minutes and 30 minutes of EGF stimulation, but the protein levels of p-ERK weren't changed significantly. Conclusion EGF raised the levels of p-EGFR, p-Akt protein of the transfected GL15 cell line, but don't changed the level of p-ERK protein.