| Background and objectiveThe expressions of receptor tyrosine kinase (EGFR) and Lrigl in lung adeno-carcinoma tissue are observed; while the relationship between the expressions of EGFR and Lrigl and clinical pathological characteristics (histological type, TNM stage, differentiated degree, gender and age) and prognosis in patients with lung adeno-carcinoma is discussed.Experiment method(1) The expression of receptor tyrosine kinase (EGFR) in lung adenocarcinoma tissue and the clinical significanceThe expression of EGFR in lung adenocarcinoma tissues and matched normal lung tissue adjacent to the adenocarcinoma are tested with immuno-histochemical staining in 75 patients with lung adenocarcinoma. All cases are enrolled from excised specimens of lung adenocarcinoma inpatients who were treated in the Department of Thoracic Surgery of Cixi People’s Hospital from 2008 to 2010. According to staining intensity (i.e. negative, weakly positive, moderately positive and strongly positive with a score from 0 to 3)and the ratio of positive staining cells (i.e.≤10%, 11%-30%,31%-50% and >50% with a score from 0 to 3), when the sum of the two scores is 3 or above 3,the case is evaluated as high expression of EGFR (EGFR High);and when the sum is below 3,it is evaluated as low expression (EGFR Low).(2) The expression of Lrigl in lung adenocarcinoma tissue and the clinical significanceThe expression of Lrig1 in lung adenocarcinoma tissues and matched normal lung tissue adjacent to the adenocarcinoma are tested with immunohistochemical staining in 75 patients with lung adenocarcinoma. According to staining intensity (i.e. negative, weakly positive, moderately positive and strongly positive with a score from 0 to 3)and the ratio of positive staining cells (i.e.<10%,11%-30%,31%-50% and>50% with a score from 0 to 3),when the sum of the two scores is 3 or above 3, the case is evaluated as high expression of Lrigl (Lrig1High);and when the sum is below 3,it is evaluated as low expression (LriglLow).The software package of STATA 13.0 is adopted to perform statistical analysis. χ2 test is adopted to perform significance analysis for inter-group data. P below 0.05 is defined as statistical significance. Log-rank test is adopted to analyze the relationship between EGFR and Lrigl expressions and prognosis; Kaplan-Meier method is adopted for survival analysis.ResultThe manifestations of high expressions of EGFR are brown-yellow or dark brown particles appeared in cytoplasm and membrane of cancer cells.The ratio of EGFR high expression in lung adenocarcinoma tissue is 64.0%(48/75), while the ratio of EGFR high expression in normal lung tissue adjacent to the adenocarcinoma is 21.3%(16/75); the difference is significant (p<0.001). It is suggested by further analysis on the relationship between EGFR expression and clinical pathologic factors of lung adenocarcinoma that there is no correlation between the expression and age, gender, differentiated degree, T stage and pathological stage (p>0.05;p=0.307,0.985,0.707, 0.855 and 0.089).The manifestations of high expressions of Lrig1 are brown-yellow or dark brown particles appeared in cytoplasm and membrane of cancer cells. The ratio of Lrigl high expression in lung adenocarcinoma tissue is 60.0%(45/75), while the ratio of Lrigl high expression in normal lung tissue adjacent to the adenocarcinoma is 84.0%(63/75); the difference is significant (p<0.001).It is suggested by further analysis on the relationship between Lrigl expression and clinical pathologic factors of lung adenocarcinoma that there is no correlation between the expression and age, gender, differentiated degree, T stage and pathological stage (p>0.05).Test on EGFR and Lrig1 is combined to analyze the relationship between expression feature and clinical pathological features in 75 cases of lung adenocarcinoma tissues. EGFRLowLriglLow accounts for 14.7%, and EGFRHighLrig1Low, EGFRLow Lrig1High, and EGFRHighLrig1High for 22.7%,18.7% and 41.3%. It is suggested that there is no correlation between expression feature and age, gender, differentiated degree and T stage (p>0.05;p= 0.714,0.997,0.242 and 0.685); while the expression feature is correlated with pathological stage (p<0.05;p=0.046).The shortest survival time among the 75 patients was 3 months. There are 24 death cases in 48 lung adenocarcinoma patients with EGFR high expression, there are 7 death cases in 27 lung adenocarcinoma patients with EGFR low expression, it is indicated that there is a correlation between EGFR expression and the prognosis in patients with lung adenocarcinoma (p<0.05).In 45 lung adenocarcinoma patients with Lrigl high expression, the death case amount is 16;while there are 18 death cases in 30 lung adenocarcinoma patients with Lrigl low expression; it is indicated that there is a correlation between Lrigl expression and the prognosis in patients with lung adenocarcinoma (p<0.05). It is suggested by combining EGFR and Lrigl tests that the prognosis in patient with EGFRHighLriglLow is poorest and that there are statistical differences of survival times between patients with EGFRLowLrig1Low, EGFRHigh Lrig1Low, EGFRLowLrig1High and EGFRHighLriglHigh (p<0.05).ConclusionThe rate of high EGFR expression in lung adenocarcinoma tissues is significantly higher than in normal tissues adjacent to the adenocarcinoma. There is no correlation between the high expression and age, gender, differentiated degree, T stage and pathological stage in patients with lung adenocarcinoma; while poorer clinical prognosis is indicated by EGFR high expression.The rate of high Lrigl expression in lung adenocarcinoma tissues is significantly lower than in normal tissues adjacent to the adenocarcinoma. There is no correlation between the high expression and age, gender, differentiated degree, T stage and pathological stage in patients with lung adenocarcinoma; while poorer clinical prognosis is indicated by Lrigl low expression.It is showed by the combination of tests on expressions of EGFR and Lirg1 that there is no correlation between the combination and age, gender, differentiated degree and T stage, but that the combination correlates with pathological stage. The prognosis in patient with EGFRHigh LriglLow is poorest. Also, there are statistical differences of survival times between patients with EGFRLowLrig1Low, EGFRHighLrig1Low, EGFRLow Lrig1High and EGFRHighLrig1High. |
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