Detection Of BRAF Mutation And Its Clinical Significance In Thyroid Cancer

Objectives:To investigate the situation of BRAF mutation in benign and malignant thyroid tumors and to explore its relationship with clinicopathological features in papillary thyroid cancer (PTC).Methods:Polymerase chain reaction (PCR) and gene sequencing were performed to detect BRAF mutation in 104 cases of benign and malignant thyroid tissues.Results:In 104 cases, BRAF mutation was detected in 46 out of 79 PTC cases with a detection rate of 58.2%.It was negative in other kinds of thyroid cancer and benign thyroid diseases. According to the clinicopathologic date, the difference between BRAF mutation in PTC and clinicopathologic features, including clinical stage, extra-thyroidal extension and lymph nodal metastasis, was statistically significant (P<0.05), but not with age, tender and tumor size(P>0.05).Conclusions:BRAF mutation was an important and common molecular marker of PTC and had a significant correlation with the presence of clinical stage, extra-thyroidal extension and lymph nodal metastasis of PTC.