Research Of Thiophenes And Phenolic Acids In Flaveria Bidentis (L) Kuntze

In this article, the extraction techniques of thiophenes and phenolic acids from Flaveria bidentis (L) Kuntze were investigated, including determination, the optimum solution in extraction, separation and purification. The data was supported in order to make use of this invasive plant.This thesis carried out a study on the extraction techniques of thiophenes and phenolic acids. The optimum extraction techniques were determined by the results of detection. The optimum extraction condition of thiophenes by refluxing is as follows:petroleum ether, ratio of material and fluid:1:10(w/v), once refluxing under 65℃, extracting time:1h. The optimum extraction condition of phenolic acids by refluxing is as follows:solution of NaOH at pH=10, ratio of material and fluid:1:15(w/v), twice refluxing under 95℃, extracting time:1h, filtrate was adjusted to pH=3 using 1mol·L-1 HCl.High-speed counter-current chromatography (HSCCC), being a support-free liquid-liquid partition method, eliminates irreversible adsorption of sample onto the solid support, and has been widely used in preparative separation of natural products. In present study, the two phase solvent system of HSCCC composed of hexane-acetonitrile=1:1(v/v), which has been selected by semi-preparative HSCCC.2.2 mg 5-(3-buten-1-ynyl)-2,2'-bithienyl,5.2 mg a-terthienyl and 4.3 mg 5-(3-penten-1-ynyl)-2,2'-bithienyl with the purity of 90.2%,99.9% and 92.1% respectively, were produced from 265.6 mg crude extract, and 5-(3-penten-1-ynyl)-2,2'-bithienyl was isolated from Flaveria bidentis (L) Kuntze for the first time. The structures of the separated compounds were identified by EI-MS,1HNMR and 13CNMR.By using Petri dishes filter method, part of bioassay of allelochemicals in Flaveria bidentis (L) Kuntze were proceeded in order to study its allelopathy.