| Objective: To study expression regarding TGF-β1, MMP-8 in transversalis fascia of patients with inguinal hernia, and the content and proportion of type I/Ⅲcollagen in inguinal conjoint tendon to further explore the mechanism of inguinal hernia.Methods: 30 cases with direct inguinal hernia were selected randomly to form direct inguinal hernia group, and 30 cases with indirect inguinal hernia were selected randomly to form indirect inguinal hernia group, and 30 non-hernia patients to form normal control group. Through immunohistochemistry (PV), the expression were detected regarding TGF-β1, MMP-8 in transversalis fascia, and the content and proportion of type I/Ⅲcollagen in inguinal conjoint tendon.Results: 1. The expression levels regarding TGF-β1 in transversalis fascia: 602.9±33.5 in normal control group, 562.8±28.3 in indirect inguinal hernia group, and 540.3±26.8 in direct inguinal hernia group with P<0.05 between any two groups. The expression levels regarding TGF-β1 in transversalis fascia were listed in order: normal control group>indirect inguinal hernia group>direct inguinal hernia group.2. The expression levels regarding MMP-8 in transversalis fascia: 194.2±25.6 in normal control group, 229.2±30.8 in indirect inguinal hernia group, 245.0±30.4 in direct inguinal hernia group with P<0.05 between any two groups. The expression levels regarding MMP-8 in transversalis fascia were listed in order: normal control group<indirect inguinal hernia group<direct inguinal hernia group.3. Negative correlation was observed between TGF-β1 and MMP-8 with correlation coefficient of -0.796, P<0.05. 4. The expression levels regarding Type I collagen in inguinal conjoint tendon of each group: 1810.6±94.1 in normal control group, 1510.5±88.6 in indirect inguinal hernia group, 1340.0±87.9 in direct inguinal hernia group with P<0.05 between any two groups. The expression levels regarding Type I collagen in inguinal conjoint tendon of each group were listed in order: normal control group>indirect inguinal hernia group>direct inguinal hernia group.5. The expression levels regarding TypeⅢcollagen in inguinal conjoint tendon of each group: 544.5±29.6 in normal control group, 664.4±31.5 in indirect inguinal hernia group, 756.6±31.2 in direct inguinal hernia group with P<0.05 between any two groups. The expression levels regarding TypeⅢcollagen in inguinal conjoint tendon of each group were listed in order: normal control group< indirect inguinal hernia group< direct inguinal hernia group.6. The condition regarding proportion of type I/Ⅲcollagen in inguinal conjoint tendon of each group: 3.33±0.23 in normal control group, 2.27±0.08 in indirect inguinal hernia group, 1.77±0.13 in direct inguinal hernia group with P<0.05 between any two groups. The condition regarding proportion of type I/Ⅲcollagen in inguinal conjoint tendon of each group were listed in order: normal control group>indirect inguinal hernia group>direct inguinal hernia group.Conclusion: 1. TGF-β1 reduction and MMP-8 increase in transversalis fascia may accelerate the decomposition of collagen transversalis fascia and reduce the intensity of transversalis fascia, which can promote the occurrence of hernia, especially in the condition of direct inguinal hernia; Through correlation analysis of TGF-β1 and MMP-8, it was concluded that TGF-β1 may have inhibitory action on MMP-8, and normal expression of TGF-β1 can promote low expression level of MMP-8 to reduce collagen decomposition and the incidence of hernia.2. The content of type I collagen in inguinal conjoint tendon were decreased, and the content of typeⅢcollagen in inguinal conjoint tendon were increased. Type I collagen and type I/Ⅲcollagen ratio in inguinal conjoint tendon were decreased. The condition above may cause the strength of inguinal conjoint tendon decline. When abdominal pressure increases, the back wall of inguinal canal becomes much weaker and induces the occurrence of hernia, especially in the cases of direct inguinal hernia. |
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