Association Of Gene Polymorphism Of SDH Gene With Type 2 Diabetic Retinopathy

PurposeThe prevalence of Diabetic retinopathy (DR) is association with diabetes duration,onest and age. Apparently, genetic factors play an important role. The polyol pathway considered to play an important role in the development of diabetic complications.It consists of two enzymes aldose reductase (AR) and sorbitol dehydrogenase (SDH).Other studies observed that polymorphism of SDH gene was associated with type 2 diabetic retinopathy.In our work,we want to search an association between the G-888C polymorphisms of the sorbitol dehydrogenase gene promoter polymorphism rs3759890 and the occurrence and progression of type 2 DR in China southern crowd.Methods1. Collect 187 individuals with T2DM diagnosed at the Guangdong General Hospital,Guangzhou,China in 2010. Of these,69 had NDR,118 had NPDR, and 123 individuals without DR served as controls. All subjects underwent ophthalmic examination, including best corrected visual acuity, slit lamp examination, and fundus examination. according to a 2002 international clinical classification of DR standard, DR was divided into NPDR group(53 patients) and PDR group(65 patients). 2.2mL Peripheral blood samples were obtained from all subjects, EDTA K2,-80℃saved. Collect the clinical data of all subjects, including systolic (SBP), and diastolic (DBP), fasting plasftvma glucose (FPG) and glycated hemoglobin (HbA1c), low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglycerides (TG) and total cholesterol (TC).3. DNA preparation and purify to contain the purpose of G 888C polymerase chain reaction fragments.4. Compared the clinial data between NDR group and DR group, including systolic (SBP), diastolic (DBP), fasting plasma glucose (FPG), glycated hemoglobin (HbA1c), low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglycerides (TG) and total cholesterol (TC),.5. Compared the clinial data between three genetype groups including systolic (SBP), diastolic (DBP), fasting plasma glucose (FPG), glycated hemoglobin (HbAlc), low-density lipoprotein (LDL), high-density lipoprotein (HDL), triglycerides (TG) and total cholesterol (TC),.6. Polymerase chain reaction-restriction fragment length polymorphism technology (PCR-RFLP) was applied to determine the genotypes of the G-888C polymorphism, comparison group genotype and allele frequency.7. Software SPSS13.0 was applied to statistical analysis.Results1. Between DR group and NDR group clinical features comparison shows, diabetes duration of DR group (10.94±7.33a)was significant longer than that in NDR group(6.75±5.29a) (t=4.522,P=0.000), and there were no significant differences in the age, diabetes duration, HbAlc, and arterial blood pressure in two groups. 2. There were no significant differences in the age, diabetes duration, HbAlc, and arterial blood pressure among patients with three genotypes (P>0.05).3. The genotype frequencies in DR group, NDR group and normal control group were 24.6%,8.7% and 8.1% respectively, and frequencies of G allele were 42.4%,25.4% and 29.7%, showing statistically significant differences among these three groups. GG genotype and G allele frequencies were significantly higher in DR group than those NDR group and normal control group(x2=17.805 P=0.001;x2=14.047 P=0.001).4. There were no significant differences in the frequencies of GG genotype and G allele between NDR group and normal control (P>0.05) as well as proliferative DR group and nonproliferative DR group (P>0.05).ConclusionDuration of diabetes is is associated with DR.SDH G-888C gene polymorphism is associated with the onset of diabeticretinopathy in south Chinese. There were no association with development of diabeticretinopathy.