Effects Of BFGF On P-Akt (Ser473) And Fractalkine Expression In Cerebral Ischemia/Reperfusion Of Rats

Objective To study the effects of basic fibroblast growth factor (bFGF) on apoptotic neurons and the expression of p-Akt(Ser473) and Fractalkine in cerebral cortex after cerebral ischemia/reperfusion of rats, and further explore the Neuroprotective mechanism of bFGF.Methods 36 rats were randomly divided into 3 groups:sham group, ischemia/ reperfusion group and bFGF group.The model of middle cerebral arteries occlusion were performed with intraluminal filament blockade,block the middle cerebral arteries for 1 hour then reperfusion for 24 hour, Neurological signs of all rats were scored under Bederson' standard. staining of rats brain were done and the average infarct volume were calculated, Tunel method for checking number of apoptotic neurons, and the expression (immunohistochemistry,IHC) of p-Akt(Ser473) and Fractalkine.Results:1. The neurological action scores:the ischemia/reperfusion group was higher than the sham group,comparing with the ischemia/reperfusion group, the neurological action scores of bFGF group was lower (p<0.05).2. The average infarct size:the ischemia /reperfusion group was higher than the sham group, the bFGF group was lower than ischemia/reperfusion group(p<0.05).3.Number of apoptotic neurons:the ischemia/ reperfusion group was higher than the sham group,the bFGF group was lower than ischemia/reperfusion group(p<0.05).4.Expression of p-Akt(Ser473):comparing with the sham group, the expression of p-Akt(Ser473) of the ischemia/reperfusion group was lower,and the bFGF group was mainly higher than ischemia/reperfusion group(p<0.05). 5.Expression of Fractalkine:comparing with the sham group, the expression of Fractalkine in ischemia/reperfusion group was lower,and the bFGF group was higher than ischemia/ reperfusion group(p<0.05).Conclusion:1.Further evidence that p-Akt (Ser473) involved in the process of cerebral ischemia, increased its expression can be effective in reducing ischemia and reperfusion in neuronal apoptosis.2. evidence that Fractalkine involved in the process of cerebral ischemia, increased its expression can be effective in reducing ischemia and reperfusion in neuronal apoptosis.3.Improving the expression of p-Akt(Ser473) and Fractalkine may be one of the molecular mechanisms of bFGF protecting nerve cell after cerebral ischemia reperfusion injury of rat.