| Objective To study the protective effect of resveratrol (RES) on intestinal mucosal barrier in ischemia-reperfusion rats and explore the possible mechanism.Methods Twenty-four SD rats were randomly divided into three groups:①sham- operation group(SO group, n=8):Just separated the superior mesenteric artery and did not clamp it;②ischemia-reperfusion group and normal saline group(I/R group, n=8): Separated and clamped the superior mesenteric artery for 45 minutes followed by 6 hours of reperfusion,0.5ml normal saline was administered via caudal vein at 5 minutes before reperfusion was achieved by removal of the clamp;③ischemia-reperfusion and RES-treated group(RES group, n=8): Separated and clamped the superior mesenteric artery for 45 minutes followed by 6 hours of reperfusion, RES (20mg/kg) was administered via caudal vein at 5 minutes before reperfusion was achieved by removal of the clamp. All the animals were given fasting and free drinking for 12 hours before testing. The rats were anesthetized, then by median abdominal incision, separated and clamped the superior mesenteric artery for 45 minutes, followed by reperfusion was achieved by removal of the clamp. All the animals were administered normal saline (0.5ml/kg) via abdomen in order to keep the hemodynamics being stable. The rats were sacrificed after 6 hours of reperfusion. And peripheral blood was taken immediately, and then the samples were centrifugalized in 4000r pm for 20 minutes, and then got the supernatant liquid. The serum was stored in -70℃for detecting the content of diamines oxidase (DAO) and small intestine fatty acid-binding protein (IFABP).The ileocecum proximal intestine of all the rats was obtained. The apoptosis of the mucosal cells was detected by Terminal-deoxynucleotidyl transferase mediated nick end Labeling (TUNEL).And the histological examination was performed by the method of staining with haematoxylin-eosin(HE). Software SPSS16.0 was used in all statistical tests and the measurement data were presented as mean(Xˉ)±standard deviation (SD).Comparisons between 3 groups were calculated using independent sample t test, and the difference was considered to be significant if the P value was less than 0.05. Results1. The pathomorphology change of ileal tissue of each groupIn SO group, under microscope, the intestinal mucosa was completely and linedly. In I/R group, the intestinal mucosa presented dilated and exposed capillaries and denuded villi, some of villi hemorrhage was ulceration; the lamina propria was edema and infiltrated with inflammatory cells. The intestinal mucosal structure maintain intact with lifting of the pithelial layer from the lamina propria and moderate extension of the subepithelial space in RES group.2 .The change of content of DAO and IFABP in different groupsThe DAO content in serum of I/R group was significantly increased compare with that in SO group (2.92±0.30 vs 0.63±0.15, P<0.05), but it was significantly decreased compare with that in RES group (2.92±0.30 vs 1.65±0.24, P<0.05). The IFABP content in serum of I/R group was significantly increased compare with that in SO group (1443.76±174.62 vs 26.76±4.86, P<0.05), but it was significantly decreased compare with that in RES group (1443.76±174.62 vs 845.12±123.86, P<0.05).3. The apoptosis of the mucosal cells in different groupsIn SO group,there are only a few scattered apoptotic cells at the top of intestinal villi, inherent layers and the submucosa. In I/R group, the number of apoptotic cells was significantly increased compare with that in SO group (P<0.05), rang from the top to the bottom of intestinal villi, inherent layers and the submucosa. While in RES group, the apoptotic cells was significantly decreased compare with that in I/R group (P<0.05).Conclusionsl. The intestinal I/R injury can destroy the mucosa structure of small intestine,increase the intestinal permeability and the apoptosis of the mucosal cells.2. Administration of RES could alleviate the small intestinal histopathologic damage, decrease the intestinal permeability and the apoptosis of the mucosal cells, and provide protective effect on intestinal mucosal barrier in ischemia-reperfusion rats. |
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