The Influence And Mechanisms Of Mythylglyoxal On Growth And Apoptosis Of Human Ovarian Cancer Cells HO8910

OBJECTIVE: To elucidate the effects of methylglyoxal (MGO) on growth and apoptosis of human ovarian cancer cell HO8910, and investigate the potential mechanisms.METHODS: The human ovarian cancer cells HO8910 were cultured in vitro. The HO8910 cells were treated with MGO in different concentrations and times. The effect of MGO on cell growth was evaluated by MTT assay; Hoechst33258 staining assay was used to observe MGO-induced morphological changes; the flow cytometer(FCM) was used to analyze the distribution of cell cycle; the apoptosis was detected by FCM using FITC Annexin-â…¤and propidium (PI) staining; Intracellular oxidative levels were measured by FCM assay using an oxidant sensitive dye2,7-dichlorefluoresin (DCFH).RESULTS: As compared with the control group, the growth of the cells cultured with MGO was inhibited. There is a correlation between the growth inhibition induced by MGO and the concentration. The inhibition rate was increased with the increasing concentration of MGO (P<0.01) when less than 24 hours; however, When the time more than 24 hours, the largest inhibition effect was seen at 1.5mmol/L. If the concentration was over 1.5mmol/L, the inhibition rate decreased. And the growth inhibition was in a time-dependent manner (P<0.05). After the treatment of MGO for 24 hours, the cell ratio of G0/G1 phase was significantly increased (P<0.05) and S phase decreased (P<0.05), and the cell ratio of G2/M phase did not change (P>0.05). Apoptosis occurred when HO8910 cells were incubated with MGO, evidenced by morphological changes and increased proportion of Hoechst positive cells. Moreover, apoptotic cells cultured with MGO was of dose-dependent that measured by FCM (P<0.05). After the cells were incubated for 24 hours, the level of intracellular oxidation increased significantly, and this trend was of dose-dependent as well as apoptosis.CONCLUSIONS: MGO can inhibite the growth of HO8910, the mechanism is probably that the cells were arrested in G0/G1 phase by MGO. MGO could induce apoptosis in HO8910 by increasing intracellular oxidative level. Therefore, MGO could be a potent apoptosis inducer, which may have a potential for ovarian cancer treatment.