| Objectives:To objective the effects of simvastatin on the expression of the HUVEC inflammatory factor induced by high density glucose and investigate its underlying mechanism.Methods:1. HUVEC were cultured in vitro.2. There is high density glucose (20,25,30,33,35,50mmol/L) was given to the HUVEC in the RPMI-1640 culture fluid, and to cultivate for 24 hours.3. Different density of NS398(10umol/L,20umol/L)was added to two different groups according to the concentration and used to incubate HUVEC for 24 hours while stimulated by high density glucose(33mmol/L).4. Different density of simvastatin(10-5mol/L,10-6mol/L,10-7mol/L, 10-8mol/L) was added to four different groups according to the concentration and used to incubate HUVEC for 24 hours while stimulated by high density glucose(33mmol/L).5. The supernatant of each culture fluid group were collected, the vigor of HUVEC was detected by MTT chromatometry,LDH was measured using Microplate Reader,inflammatory factor such as IL-6,CRP were measured by ELISA,expression of COX-2 mRNA in HUVEC was measured by RT-PCR.Results:1. The vigor of HUVEC , which was stimulated by high density glucose(20,25,30mmol/L),was lower than the control group,But without statistical significance (P>0.05),33,35,50mmol/L were significantly lower according to the concentration (P<0.05),The HUVEC stimulated by high density glucose(33mmol/L),has significantly higher LDH values than the control group(P<0.05). 2. LDH of HUVEC stimulated by high density glucose(33mmol/L), which has been added different density of simvastatin(10-5mol/L,10-6mol/L)and different density of NS398(10umol/L,20umol/L),was significantly lower than positive control group,but the vigor of HUVEC was significantly higher (P<0.05).There was no significant difference between the Sim group(10-7mol/L,10-8mol/L) and the positive group.3. IL-6 and CRP expression of HUVEC stimulated by high density glucose(33mmol/L), which has been added different density of simvastatin(10-5mol/L,10-6mol/L)and different density of NS398(10umol/L,20umol/L) was significantly lower, comparing to the positive group, There was no significant difference between the Sim group(10-7mol/L,10-8mol/L) and the positive group.4. COX-2mRNA expression of HUVEC stimulated by high density glucose(33mmol/L), which has been added different density of simvastatin(10-5mol/L,10-6mol/L,10-7mol/L)and different density of NS398(10umol/L,20umol/L) was significantly lower than the positive group There was no significant difference between the Sim group(10-8mol/L) and the positive group.Conclusions:1. High density glucose could upregulate the COX-2 mRNA expression, induce HUVEC inflammation, and injury the HUVEC.2. Simvastatin could suppress the injury of HUVEC induced by high density glucose.The mechanism relates to it could downregulate the expression of COX-2 mRNA and suppress inflammation... |
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