The Effect Of Oocytes Age To Parthenogenetic Activation On Mouse Oocytes

Objectives This paper aims at finding a better oocytes age for mouse parthenogenetic activation, thus providing a more normalized experimental method and data for the Mouse Oocytes Parthenogenetic research, Embryo Cloning and the activation of the reconstructed nuclear transfer.Methods The experimental method of this research of combining 7% of ethanol to the Cytochalasin B final concentration is of 5 % , and then adopt the mixture to study the CD-1? oocytes age of mouse for 14h,16h,18h,20h and 22h of Oocytes Parthenogenetic activation respectively; before conducting further morphologic observation and analysis on formed embryo .Results parthenogenetic activation rate (95.42%),blastula formation rate(48.43%), hatching rate(7.30%) of oocytes age of 18hs were higher than parthenogenetic activation rate (86.40%,91.58%),blastula formation rate(10.74%,13.13%) of oocytes age of 16hs and 20hs;16hs and 20hs of oocytes age parthenogenetic activation rate,blastula formation rate were higher than 14hs and 22hs of oocytes age parthenogenetic activation rate(83.37%,88.91%),blastula formation rate(7.12%,9.60%); There is no significant difference between oocytes age of 16hs and 20hs group oocytes age activation rate and blastocyst formation rate, also,there is no significant difference between oocytes age of 14hs and 22hs group oocytes activation rate and blastocyst rate .Compared the five groups there are major differences(p<0.05) between 18hs of oocytes age group and the other four groups in parthenogenetic activation rate,blastula formation rate and the hatching rate .Conclusions As mentione above of the activation in the groups of oocytes age: (1)_The ethanol and cytochalasin B combined activation 18h of oocytes age mice has the obvious advantages in terms of cleavage rate, blastula formation and hatching. (2)_From the general morphology, 18hs of oocytes age rate shows that activated embryo develops normally and the embryo activated by ethanol group has more potential of future development, which is the key point of the activation. (3)_ Parthenogenetic activation and the following development are affected by factors such as, dosage of COS, method of activation, the system of cultivate ng and the proficiency of operating.