The Expression Of Calcitonin Gene-related Peptide In Bone Healing Area After The Immediate Implant Preparaed.

[Objective] Established the rat alveolar bone injured models of the oral immediately implant bed preparation , which aim was to observe the characteristics of calcitonin gene related peptide expression in the alveolar bone healing process by HE and immunohistochemistry assay. We discussed the experession of calcitonin gene-related peptidein alveolar bone healings and remodeling , and investigated the peptide action mechanism on alveolar surgery and oral implant.[Methods] 16 healthy adult male Wistar rats were randomly divided into 4 groups , there were 4 each group. Established the rat alveolar bone injured models of the oral Immediately implant bed preparation , and collected specimens group after 4,7,14,21days. The bone injured models of immediate implantation fossa preparation : Rats were weighed and anesthetized by 0.4% sodium pentobarbital intraperitoneal injection at a dose of 1 ml per gramme . Under sterile conditions, pulled out the first molar by dental tweezers , and then prepared implants nest in proper depth and diameter with a small impact drilling . Taken a short alveolar bone (4 mm×4 mm×4 mm) in the model preparation area as specimens respectively after 4,7,14,21days. all specimens were fixed 24 hours by 10% formaldehyde solution , And decalcified by neutral EDTA , dewaxed , embedded, sliced,SP stained, HE stained . Finally ,counted immunoreactive cells under the light microscope , captured images, measured cumulative optical density by Image-Pro plus 6.0 system, done statistical analysis .[Results] 1. Established Models successfully: 16 rats were well during the procedure of implanting hole shape, and the surgical operation areas had no significant swelling and infection .2. HE staining showed that more fibrous tissuse in bone repair areas at the 4th day and the 7th day; The repair bone cell were most activtive in bone repair areas at the 4th day and the 7th day, which nuclei were deeply stained; There had osteoblasts and osteoclasts apppeared at the edge of bone repair areas. But no in the central areas .3. Osseous tissue immunohistochemistry staining show : masculine cells'endochylema were stained ; the ripe osseous cells weren't stained , but the osteoblast were stained in the margin of osseous repair area; 7 days after established models , the fibroblast were stained in the center of osseous repair area , the positive cell amount was the most and stain intensity was the the most strong in this stage ,which compared to the bone healing tissue of implantation area in other different time . Rank sum test of 4 groups , the amount of CGRP positive cells and IOD came to the peak in the seventh day , and it was have the covariance learns meaning .[Conclusions] 1.There was higher CGRP expression in bone healing area , meanwhile osteoblast-like cell higher proliferation (the critical period of bone cell formation). CGRP alveolar bone were significantly downregulated in the normal, proved the opposite point of view , which CGRP showed positive active only when the bone remodeling cells'function was active. It may be an important factor to promote bone formation and to inhibit bone resorption.2.The peak expression of CGRP appeared at the phase ,which the fibrous callus change to the bony callus, and the phase was also important at the fibroblasts were induced to express bone formation .It suggested that CGRP may be an important factor for inducing fibroblasts to express the bone formation function .3. CGRP showed strong positive experssion in the cytoplasm of former osteoblasts and fibroblasts of expressing bone formation function . we consideried the positive expression of CGRP not only a sign of the former osteoblasts formation and proliferation , but also inducing fibroblast expressing bone formation function .