Chemical Constituents Of Streptomyces Xiamenensis Sp. Nov From Mangrove Soil

The metabolists of mangrove microorganism show diversities in structure and activity because of particularity in their living environment.In order to investigate the metabolists with novel structure and potential bioactivity,we have systematically investigated the chemical constituents of the microorganism Streptomyces xiamenensis sp.nov.,which was separated from mangrove soil in FuJian province.On the basic of silica gel column chromatography,PTLC,Sephadex LH-20 column chromatography,ODS column chromatography,HPLC,fourty-three pure compounds were obtained from the mangrove soil microorganism,guided 1H-NMR spectrum and DAD-HPLC analysis.thirty-seven structures were determined spectroscopic analysis,including NMR,MS,IR,UV spectra,which are 2-Butenoic acid (1), Benzeneacetic acid (2), Benzoic acid (3),1 H-Indole-7-methanol (4),1-Butanone,1-(4-hydroxyphenyl) (5), Benzoic acid,4-methoxy-, hydrochloride (6),2-Propenoic acid,3-phenyl (7), Cyclo(leucyl-proline (8), Cyclo(L-Phe-Pro) (9),7-methyl-1H-purin-6(7H)-one (10), Cyclo(L-Phe-Pro) ( 11),2H-Purin-2-yl,2-amino-1,3,6,9-tetrahydro-6-oxo-9- -D-ribofuranosyl-(12),4-hydrox ybenzoic acid(13), Fumalic acid(14), (S)-2',3'-dihydroxy-1-(1H-indol-3-yl)propan-1-one(15 ), (R)-2',3'-dihydroxy-1-(1 H-indol-3-yl)propan-1-one(16), Benzopyran derivatives(17), 2,4-dihydroxybenzoic acid(19), cyclo[Pro-Val](23),2'-methyl-1H-indole-3-carboxylic acid(24), methyl 4-hydroxybenzoate(25),3-Pyridineethanol(26), methyl 2-hydroxy-3-(1H-indol-3-yl)propanoate(27), cyclo[Gly-Leu](28), N-phenethylacetamide(29), cyclo[Leu-Ala](30), cyclo[Val-Leu](31), cyclo[Tyr-Pro](32), cyclo[Ala-IIe](33), cyclo[Ala-Val](34), cyclo[Trp-Ala](35), cyclo[Trp-Ser](38), cyclo[Trp-Val](39),1,4-Benzenediol (40), cyclo[Trp-Pro](41),1-Butanone,2'-hydroxy-1-(1H-indol-3-yl)-(42),3H-Indole-3-carboxylic acid (43)。In a word,there were two problem needing to resolved.Firstly,amount of the secondary metabolists that microorganism produced was very little,so it was difficult in screening.Secondly,the range of screening was so limited.In order to search special activities of novel compounds and provide guiding compounds for studies on new medicine, we should take better advantage of mangrove soil microorganism,such as enlarging fermentation scale,optimize culture method to produce secondary metabolists directionally and screen in highly efficient and wide-ranging way.