Effect Of THH On Expressions Of HIF-1α In Rats With Collagen Ⅱ-induced Arthritis

Objective: To investigate the effect of different doses of THH on expressions of HIF-1a In rats with collagenⅡ-induced arthritis(CIA), and discusses the mechanism.Methods: Using sixty wistar rats(half male and half female ). Including 10 as control group, the remaining 50 preparation for CIA model. The CIA model groups were intradermally sensitized under anesthesia with 0.5ml bovine type II collagen, emulsified in cold Freund's incomplete adjuvant. 14days later, the rats received an intradermal booster injection of equivalent volume used for sensitization. After the first immune for 28days, The CIA model groups randomly divided into five groups: one model control group, one treatment group of dexamethasone, one treatment group of high-dose THH(400mg/kg/d), one treatment group of moderate-dose THH(300mg/kg/d), one treatment group of low-dose THH(200 mg/kg/d). And then begin to treatment for 30ds, dynamic viewing the rat's physical changes and the joint inflammation. At the end, observe the pathological changes of the joints with HE staining, detect the expression of HIF-1αwith the method of RT - PCR and immunohistochemical. To do the research of the THH ' effect to the expressions of HIF-1a In rats with collagenⅡ-induced arthritis(CIA), and discusses the mechanism.Results: After the treatment for 30 days, HE staining reveal that the model control group are full of layers of synovial cells, a large number of inflammatory cells,and vascular pannus is formed; But between the groups of treatment, dexamethasone works best, inflammatory cells rarely exist, only normal 2-3 layers of the synovial cells; the therapeutic effect of moderate-dose THH is best, synovial layer in normal level, inflammation and vascular "pannus are basic disappeared. Immunohistochemical shows every treatment group is lower protein expression of HIF-1a in joint synovial organization in comparison of the model control group. PT-PCR results show that each treatment group's expression of HIF-1a mRNA in peripheral blood is decreased obviously.Conclusion: HE dyeing, immunohistochemical and RT-PCR results confirmed dexamethasone and THH for CIA model obvious curative effect. For its synovial organizations proteins and peripheral blood mRNA, the expression of HIF-1a has obvious inhibition. Point out that HIF-1a plays an important role in the treatment of THH in CIA. This study not only for the clinical treatment with THH in RA provides an effective treatment mechanism, But also provide broad application prospects for clinical with THH treatment in RA.