The Diagnosis Of Keloid By Use Of Multiphoton Laser Scanning Microscopy

Based on two-photon excited fluorescence (TPEF) and second harmonic generation(SHG), multiphoton laser scanning microscopy(MPLSM)gains an advantage over conventional technologies and can provide the considerable information on pathology and clinic diagnosis. In this thesis, the technology was used in clinical diagnosis of scar type, focusing on keloid. The morphological differences from the dermal layer of keloid, hypertrophic scars, normal scars and the boundary between normal skin and scar tissue firstly investigated. Collagen and elastin volume density was performed to differentiate the three scar tissues. Then, by using SHG, the collagen propria of keloid skin's different parts of the dermal layers were investigated. And the collagen orientation index was used for quantitative description. Our data indicated that the differences of these developed parameters from the three scar tissue type were significant and they were able to be used as indicators to diagnose scar tissue. With the advent of clinical portability of typical MPLSM, this technique has the potential in vivo to clinical diagnosis of scar tissue non-invasively as well a monitoring the therapy response.