| Background: Chronic obstructive pulmonary disease (COPD) is characterized by airflow limitation that is not fully reversible. The airflow limitation is usually both progressive and associated with an abnormal inflammatory response of the lungs to noxious particles or gases. At present, a large number of researcher had single researched from aspects of liver function, renal function, immunological function or blood routine, but only a small part had researched about the simultaneous determination and aggregate analysis of hematological indexes, genetic polymorphism and susceptibility loci. The research about the correlation between laboratory hematological indexes and genes are scarcely reported.Objective: Genetic polymorphism of D8S1179,D21S11,D7S820,CSF1P0,D3S1358,D5S818,D13S317,D16S539,D2S1338,D19S433,VWA,D12S391,D18S51,D6S1043 and FGA in 40 patients with moderate and severe COPD were analyzed by use of the second generation DNA short tandem repeat (STR, it is also called microsatellite DNA). The correlation between laboratory hematological index and 15 STR gene loci were analyzed to explore the STR gene loci which relate to COPD, and investigate whether a correlation exists between the hematological index and STR gene locus for further information of possible progressive mechanism of COPD.Methods:1. Indexes such as blood routine (WBC,NEU,LYM,RBC,HGB,PLT) , biochemical index (TP,ALB,BUN,CRE,TG,CHE,HDL-C,LDL-C) and immunological index (IgA,IgG,IgM,C3,C4,hs-CRP) were measured in 40 cases of moderate and severe COPD group (n=40) and 20 cases of healthy control group (n1=20) by blood cell analyzer, biochemical analyzer and ELISA reader.2. 40 cases of moderate and severe COPD group (n=40) and 100 cases of healthy control group (n2=100) underwent STR detection respectively.Results:1.The difference of frequency distribution of 15 loci in COPD group and control group: D2S1338-25 and FGA26 of COPD group were significantly decreased; While D12S391-19.3 and FGA20.2 of COPD group were significantly increased.There was significantly difference between the two groups, the difference had statistical significance. (p<0.05)2.The difference of hematological index in COPD group and control group: WBC, NEU, PLT, GLU, CRE, C4, hs-CRP were significantly increased and HGB, ALB, TG, IgA were significantly decreased,the difference had statistical significance( P<0.05). While BUN, CHE, HDL, LDL, IgM were increased and LYM,RBC,TP,C3,IgG were decreased, there was no significantly differences and no statistical significance (p>0.05).3.The correlation between detection results of blood routine indexes and gene locus: the results of WBC and NEU were significantly correlated with loci of VWA 16 and D19S433-13; the result of LYM was significantly correlated with loci of FGA20.2, D6S1043-18, VWA 19, and D7S820-9; the result of RBC was significantly correlated with loci of CSF1P0-11, D7S820-9 and D8S1179-15; the result of HGB was significantly correlated with loci of D18S1179-15, D18S51-17, CSF1P0-11, D7S820-8, D8S1179-13 and D8S1179-15; the result of PLT was significantly correlated with loci of D18S1179-19, D16S569-11 and D13S317-12. There was statistical significance (p<0.05)4. The correlation between detection results of biochemical indexes and gene locus: the result of GLU was significantly correlated with loci of D7S820-10,CSF1P0-14,VWA 17,D6S1043-13,FGA20.2; the result of TP was significantly correlated with locus of D19S433-14.2; the result of ALB was significantly correlated with loci of D19S433-14.2 and FGA20.2; the result of BUN was significantly correlated with loci of D21S11-32.2 and D18S51-16; the result of CRE was significantly correlated with loci of D18S51-18; the result of CHE was significantly correlated with locus of D7S820-10; the result of HDL was significantly correlated with loci of D21S11-33.2, D3S1358-16, D16S539-11 and D16S539-12; the result of LDL was significantly correlated with locus of D5S818-13.There was statistical significance (p<0.05)5.The correlation between detection results of immunological indexes and gene locus: the result of IgM was significantly correlated with loci of D8S1179-12 and D19S433-16.2; the result of IgG was significantly correlated with locus of FGA21; the result of hS-CRP was significantly correlated with locus of FGA20.2. There was statistical significance (p<0.05)Conclusion:1.The detection results of hematological indexes in patients with COPD reflects the changes of liver function, renal function, immunologic function, blood glucose and lipid metabolism to some extent. Such a conclusion may be advantageously used in guiding the occurrence, development and prognosis evaluation of COPD.2. It was presumed that there may have COPD dangerous STR nearby FGA20.2 and D12S391-19.3, and there may have COPD protective STR nearby D2S1338-25 and FGA26.3. It was presumed that there may have the some relevance between the changes of laboratory hematological index, biochemical index and immunological index. The individual difference may be genetically determined. |
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