The Associations Of V227A Polymorphism Of The PPAR Alpha Gene With Early-onset Type 2 Diabetes Mellitus

Objective: To explore the correlations of PPARαV227A polyphism in early-onset T2DM in dalian of China.Methods: A total of 496(244 men and 252 women ) T2DM subjects were enrolled and divided into two groups: early-onset and late-onset groups which were defined as diagnosed at≤40 and >40 years old. Early-onset T2DM subjects 206(126 men and 80 women) average age was 36.33±4.02 years old. Late-onset T2DM subjects 290(118 men and 172 women) average age was 56.36±9.56 years old.Normal control group subjects were composed of 248 healthy people (112 men and 136 women).Extracted peripheral blood white blood cell genomic DNA to detect PPARαV227A polymorphism.PCR-direct sequencing was employed to detect the PPARa V227A gene mutation in two groups and frequencies of genotype and allele were observed, and the clinical characteristics of mutation gene carriers were investigated.Results:①The fasting plasma glucose(FPG), triglyceride (TG), uric acid (UA) were higher in early-onset T2DM group than in late-onset T2DM group.②The overall frequencies ofVV VA AA in early-onset T2DM group and in late-onset T2DM group were 92.2%,7.8%, 0%, and 83.1 %, 16.9%,0%. In normal control group were85.9%, 12.9%,1.2%. The V227 allele frequency was 92.2%, and A227allele frequency was 7.8%in early-onset T2DM group,and the V227allele frequency was 83.1% and A227allele frequency was 16.9%in late-onset T2DM group. There were statistical differences in the gene type frequency and allele frequency distribution between the two groups.③In early-onset T2DM group A227allele had lower total cholesterol (TC),Apo-B than V227allele.Conclusion:①The early-onset T2DM group had poorer blood glucose control, more severe dyslipidemia and higher uric acid when compared with the late-onset T2DM group.②In the early-onset T2DM group PPARαA227allele frequency was lower than in the late-onset T2DM group.③A227 variation was possibly protective factor for dyslipidemia. PPARαgene V227A polymorphism reduceded the risk of having early on-set T2DM by reducing the levels of TC and Apo-B.④PPARαgene V227A polymorphism was associated with earlyon-set T2DM dyslipidemia.It was a protective factor of dyslipidemia.