| Objective: To explore the pathogenesis of asthma, observe the dynamic changes of serum IL-16 in children with asthma,and present the significance of IL-16 in asthma pathogenesis.Methods: The research subjects were divided into three groups,i.e. asthma attack group, paracmastic group and the normal group. The cases are all asthma children and the normal group is the children taking physical examination in our hospital over the same period. Case matched criterion: the diagnosis is in line with diagnostic criteria for asthma childhood set by the national pediatrics asthma coordinating group in 2008. Attack group belongs to the onset group not using hormones 2 weeks ago bursting out the symptoms of ploypnea, cough, chest distress due to exposure to allergens and other irritants or improper treatment or having the features of a sharp increase in the original symptoms accompanied with dyspnoea, reduced expiratory gas flow. Paracmastic group are the attack cases applications ofβ2 agonists or normal applications of GINA program for long-term inhalation of steroids whose symptoms and physical signs disappear and pulmonary function recovers to the level before the onset of acute pulmonary after 8 to 12 weeks. Experiment Methods: Examine the mass concentration of IL-16 in serum by the means of Double-Sandwich ELISA. Carry out the homogeneity test of variance and variance analysis to three groups of samples and make use of t-test to means comparison for significance test. All data are processed through SPSS17.0 statistical package.Results: Three groups'gender, age structures adopt chi square test after the test of normality. The age distribution takes t-test and the test results are descript in the form of mean±standard deviation ( X±s). The difference comparisons of gender structure and average age of three groups of children showed: there is no significant difference on three groups of children'gender structure and average age (P >0.05) which suggests that three groups are comparable. The concentration levels of serum IL-16 of attack group, paracmastic group and normal group are in the standard normal distribution of which 3 samples data of paracmastic group was lost which can be negligible. Three sets of data were consistent with variance analysis criterion and the variance analysis results showed that comparisons between the three groups were F= 26.542, P <0.01, having statistical significance which indicated that there were significant differences between three sets of samples. Because the results of homogeneity analysis of variance showed that there was variance heterogeneity in every group, so making use of approximation test method (Dunnett Method T3) conducted pairwise comparisons among three groups. The obtained results are as follows: the concentrations of serum IL-16 of both attack group and paracmastic group were higher than the normal group, both were P <0.01 having significantly statistical significance while the concentration of IL-16 of attack group was higher than paracmastic group, P <0.01, having significantly statistical significance.Conclusion: The concentrations of serum IL-16 of attack group and paracmastic group were obviously higher than the normal group, and the IL-16 level of attack group significantly decreased after treatment, but still higher than the normal group. After analyzing and reviewing literatures, conclusions are as follows:1. The concentration of IL-16 of attack stage and paracmastic stage was higher than the normal group. IL-16 is an important cytokine of asthma inflammatory reaction, it plays an important role in the process of asthma.2. The concentration of IL-16 of attack stage was significantly higher than paracmastic stage. IL-16 plays the role of positive regulation in the asthma pathogenesis, and providing the basis of immunity and therapeutic intervention in early clinical treatment.3. IL-16 can promote IgE synthesis, and induce airway hyperreactivity. |
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