The Correlation Analysis Between CD90 , Collagen-Ⅰ , Collagen- Ⅳ Expression And The TGF-β TypeⅠ,typeⅡ Receptor Expression In Hypertrophic Scars

OBJECTIVE: To further explore the pathogenesis of hypertrophic scars, CD90,Collagen-Ⅰ,Collagen-Ⅳand TGF-βtypeⅠ,typeⅡreceptor expression of fibroblasts in abnormal scars and normal skin was undertaken by immunohistochemistry. The differences of CD90 expression,Collagen-Ⅰ,Collagen-Ⅳexpression,TGF-βtypeⅠ,typeⅡreceptor expression and biological characteristics of fibroblasts in abnormal scars and normal skin were investigated. The expression of CD90 and its correlation with TGF-βtypeⅠ,typeⅡreceptor expression in peripheral area of hypertrophic scar was analyzed. The expressions of Collagen-Ⅰ,Collagen-Ⅳa nd their correlations with TGF-βtypeⅠ,typeⅡreceptor expression in peripheral area of hypertrophic scar were also analyzed.METHODS:①The 21 hypertrophic scars from operations were divided into central area of hypertrophic scar group (HTS, n=21), peripheral area of hypertrophic scar group (n=21) and normal skin group (NS, n=21).②Two pathologists checked all the tissue sections with haematoxylin-eosin dying in order to support the clinical diagnosis. The paraffin block of tissue chips consisted of one core taken from each of the central area of hypertrophic scar group, peripheral area of hypertrophic scar group and normal skin group. Each paraffin block of tissue chips including 63 points of the tissue was put into array as 9×7.③Cut the paraffin block of tissue chips into five pieces of tissue chips. The specimens of tissue chips were detected for the expressions of CD90,Collagen-Ⅰ,Collagen-Ⅳand TGF-βtypeⅠ,typeⅡreceptor by immunohistochemical staining in peripheral,central areas of hypertrophic scar groups and in normal skin group. All the results were analyzed statistically.④The correlation of CD90 expression with that of TGF-βtypeⅠ,typeⅡreceptor was determined by means of statistical analysis in peripheral area of hypertrophic scar group. The correlations of Collagen-Ⅰ,Collagen-Ⅳexpression with that of TGF-βtypeⅠ,typeⅡreceptor were also determined by means of statistical analysis in peripheral area of hypertrophic scar group.RESULTS:①Tissue chips containing hypertrophic scars and normal skin were constructed, the correlation analysis of multiple factors got well effect in immunohistochemical detection.②Immunohistochemistry staining showed that 17 cases had CD90 positive phenotypic fibroblasts in 21 cases of hypertrophic scar group, 13 cases in peripheral area of hypertrophic scar group, and 4 cases in normal skin group. They had a significantly statistical difference (P<0.05) in comparison with that of normal skin group.③Collagen-Ⅰhad high expressions both in peripheral and central areas of hypertrophic scars groups, they had a significantly statistical difference (P<0.05) in comparison with normal skin group. Both peripheral and central areas of hypertrophic scars showed as collagen-Ⅰ, Collagen-Ⅳhad rarely expressions both in central areas of hypertrophic scars groups and in normal skin group. They had a significantly statistical difference (P<0.05) in comparison with peripheral area of hypertrophic scars group, while most normal skin group showed as collagen-Ⅳ.④TGF-βtypeⅠreceptor rarely expressed in central area of hypertrophic scar group, but they had varied expressions in peripheral area of hypertrophic scars groups, The difference between these two groups was of statistical significance (P<0.05). TGF-βtypeⅡreceptor had high expressions both in peripheral and in central areas of hypertrophic scars groups (P>0.05). TGF-βtypeⅠa nd typeⅡreceptor rarely expressed in normal skin group, they had a significantly statistical difference (P<0.05) in comparison with that of peripheral and central areas of hypertrophic scars groups.⑤Statistical analysis could verify the positive correlation of CD90 expression with that of TGF-βtypeⅠand typeⅡreceptor in peripheral area of hypertrophic scar group.⑥The expression of TGF-βtypeⅠreceptor has correlation with that of collagen-Ⅰand collagen-Ⅳin peripheral of hypertrophic scar group (P<0.05). The expression of TGF-βtypeⅡreceptor also has correlation with that of collagen-Ⅰand collagen-Ⅳin peripheral of hypertrophic scar group (P<0.05).CONCLUSIONS:①Tissue chips are high efficiency and well controlled in quality for multiple factors'investigation. It can be further used in the study of hypertrophic scars.②Fibroblasts expressing CD90 in hypertrophic scars might be some phenotypes specifically related to scar formation.③The variety of composition and distribution of collagen-Ⅰand collagen-Ⅳin hypertrophic scars plays an important role in the formation and development of hypertrophic scarring.④The findings of increased TGF-βtypeⅠ,typeⅡreceptor indicated that they may participate and play an important role in the formation of hypertrophic scars, TGF-βtypeⅠreceptor had high expression in peripheral areas of hypertrophic scars, so it was of great clinical significance for the prevention and treatment of hypertrophic scars, and the peripheral area would be emphasized in the clinic work.⑤The expression of CD90 have positive correlation with that of TGF-βtypeⅠand typeⅡreceptor in peripheral area of hypertrophic scar, they might have some synergistic relationships in hypertrophic scarring.⑥Collagen-Ⅰand collagen-Ⅳmight have some synergistic relationships with TGF-βtypeⅠ,typeⅡreceptor in peripheral area of hypertrophic scar.