| Objective:Through analysis the ratio changes of type I collagen and type IIIcollagen of the scar control group and laser treated group,concluded that Fractional Carbon Dioxide laser Scaar Fx can reduce theRatio of typeI Collagen and type III Collagen in rabbit ear hypertrophicscar.Methods:Firstly，buy20standard New Zealand white rabbits, then makerabbit ears bilateral ventral hypertrophic scar model. The New Zealandwhite rabbits were divided into three groups: the normal skin group(group A), scar control group (group B), laser treated group (groupC).After28days of modeled, The Group C give Fractional Carbon Dioxidelaser to be treated (Scaar Fx: Energy:60.0mJ, Frequency:250Hz, Shape:2, Size:10, Pulses:1, Density:5%).After laser treatment, all the scarlaser treatment group and the scar control group were taken0.5cm*0.5cmscar of a full-thickness specimens cuted at a interval of3days,7days,14days,21days,28days,2month,which including scar full thickness,partial cartilage layer below the normal tissue surrounding a small,neutral formalin fixed. Using HE staining to observation dermal collagenarranged and vascular hyperplasia in histological. Picrosirius-redstaining: After picric acid Sirius red staining in polarized lightmicroscope can distinguish between different fiber types: type Icollagen fiber is thick, showed yellow or red, type III collagen fibers is slender, showed green. So you can analysis the relationship betweenthe two collagen fibers and their mutual simultaneously on the same slide.Using image analysis techniques to check the ratio of type I and typeIII collagen.Results:20New Zealand white rabbits survived to complete the wholeexperiment. Firstly All rabbits were making hypertrophic scar of rabbitear model, after making a successful model then randomly divided into twogroups: scar control group (group B), laser treated group (Group C). Eachgroup have ten rabbits, normal rabbit ear skin tissue modeling processwill be achieved with neutral formalin-fixed, labeled as normal skin group(A).HE stained histological observation: The normal skin dermis has aclear hierarchy, collagen was showed bright red, and consistent withloosely arranged, rules, direction and arrangement. Scar control group(group B) can be seen a lots of fibroblasts and inflammatory cells, thecollagen fibers were distributed disorders, and collagen fibers, theextrusion of excess blood vessels, multi-cavity. The hypertrophic scartissue after Fractional Carbon Dioxide laser treated to be relativelyfewer elastic fibers. Moreover, scar collagen arranged in order, thenumber of blood vessels also decreased.Picrosirius-red staining: rabbit ear hypertrophic scar tissueafter staining shows the main characteristics of dermis is coarse typeI collagen. Scar tissue after Fractional Carbon Dioxide treatment,firstly, it showed type III collagen in dermal-epidermis junction (DEJ),as time progresses, type I collagen gradually increased, then maintaina fixed proportion in2month.The image analysis: Application SPSS18.0software for statisticalanalysis. Statistical significance was assessed by a paired t test. A p value lower than0.05was considered significant. The ratio of type I/III collagen in normal skin group (A) is1.32:1, scar control group (groupB) is much higher than the normal skin gradually(3day、7day、14day、21day、28day、2month：4.6201±1.1567:1、4.982±1.2721:1、8.2383±1.3601:1、15.6203±3.0364:1、9.3015±2.2693:1、8.7717±1.1122:1（P<0.001））; theratio of type I/III collagen is significantly lower than the ratio ofhypertrophic scar tissue ratio, in2months is close to normal skin（3day、7day、14day、21day、28day、2month：0.6151±0.6424:1、0.8817±0.5359:1、1.5233±0.0586:1、1.8517±0.0417:1、1.9433±0.9991:1、2.2067±0.6260:1（P<0.001））（Attached Table2）.Conclusion:The ratio of type I collagen and type III collagen was decreasedafter the Fractional Carbon Dioxide laser Scaar FX treatment in rabbitears hypertrophic scars, Which can provided a theoretical basis forclinical ultra-pulsed CO2Fractional Laser treatment of hypertrophicscar. |
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