Expression And Of ETS-1, HSP70 And P57kip2 And Its Significance In Giant Cell Tumor Of Bone

Objective: To study the expression of ETS-1, HSP70 and P57kip2 in giant cell tumor of bone(GCT) and its relationship with radiological classification, tumor recurrence, and pathological classification, in order to provide evidence for judging biological behavior and prognosis of GCT.Methods: Immunohistochemistry (SP) was used to investigate expression of ETS-1, HSP70 and P57kip2 in 56 cases of giant cell tumor of bone, and studied its relationship with radiological stage, tumor recurrence, and pathological grading.Results: (1) The positive expression rate of Ets-1 in GCT cases was 57.14(32/56). According to radiological stage(stage 1,2 and 3), the positive rate of ETS-1 was 39.13%(9/23) in stage 1, 64.17%(11/17) in stage 2 and 75.00%(12/16) in stage 3. The expression of Ets-1 increased by radiological stage, nevertheless the result had no statistical significance (P<0.05). The positive expression rate of Ets-1 in recurrent cases was 83.33% (15/18), higher than 44.73% (17/38) in cases without recurrence (P<0.05). According to pathological grading, the expression of Ets-1 in stage 1, 2, and 3 cases had no statistical significance. (2) The positive expression rate of HSP70 in GCT cases was 51.79%(29/56), which had significant difference according to age(P<0.05) and size of tumor(P<0.01); according to radiological stage, the positive expression rate of HSP70 in stage 1, 2 and 3 was 34.78%(7/25), 57.89%(11/19) and 91.66%(11/12) respectively, and the positive rate increased in gradient(P<0.01). The positive rate of HSP70 in recurrent cases and un-recurrent cases was 88.89% (16/18) and 34.21% (13/38) respectively, which had significant difference (P<0.01). (3) The positive rate of P57kip2 in GCT cases was 21.43% (12/56). According to radiological stage, the positive rate in stage 1, 2 and 3 was 44.00% (11/25), 5.26% (1/19) and 0.00% (0/12) respectively, and decreased in gradient (P<0.01). The positive rate in stage 1 cases had significant difference with that in stage 2 and 3(P<0.01, P<0.01), however, there was no statistical significance (P>0.05) between the positive rate of stage 2 and 3. The positive rate was 5.56% (1/18) in recurrent group and 28.95% (11/38) in un-recurrent group respectively, which had significant difference (P<0.05). (4) In the study, Spearman Correlation showed that there was a significant positive correlation between Ets-1 and HSP70, (rs=0.320, P<0.05), and there was a significant negative correlation between HSP70 and P57kip2 (rs=--0.280, P<0.05). We found there was no correlation between P57kip2 and Ets-1(rs=0.013, P>0.05).(Table 5, 6, 7).Conclusion: In GCT cases, the positive rate of HSP70 increase and P57kip2 decrease by radiological stages. All of HSP70, P57kip2 and radiological stages correlate with tumor recurrence, while has no correlation with pathological gradings. There is a positive correlation between Ets-1 and HSP70, a negative correlation between HSP70 and P57kip2 and no correlation between Ets-1 and P57kip2, which suggest that detection of Ets-1, HSP70 and P57kip2 may be an index of malignant degree, invasion, decision of tendency to recurence and selection of operation modality.