| Objective:To study the antioxidant effects of picrosideⅡon the cerebral ischemia reperfnsion injury in rats and provide theoretical basis of picrosideⅡin clinical application.Methods The MCAO/R models were establish with a suture blocking in the MCA in 100 healthy Wistar rats and were randomly divided into sham operative group, negative control group, positive control group and drug treatment group. The rats in the sham operative group were not treated, normal saline were injected into those in the negative control group, salvianic acid A sodium (10 mg·kg-1) were injected into those in the positive control group, and picrosideⅡ(10 mg·kg-1) were injected into those in the drug treatment group. The nervous behavioral function was evaluated with Bederson's test. The cerebral infarction volume was observed with tetrazolium chloride (TTC) staining. The expressions of inducible nitric oxide synthase (iNOS) and superoxide dismutase (SOD) were determined by immunohistochemical assay. The concentration of iNOS and SOD in brain tissue was determined by enzyme linked immunosorbent assay (ELISA). TUNEL positive cells were counted by immunofluoresence assay. Results The nervous behavioral malfunction was normal and there was no focus infarction in sham operative group. The expression of iNOS and SOD was weakly, the apoptotic cells were scattering in brain tissue in the sham operative group. In the negative control group, the nervous behavioral malfunction and focus infarction was higher than those in sham operative group. The expression of iNOS increased and the concentration were significantly higher than those in the sham operative group (P<0.05). but the expression of SOD decreased and the concentration were significantly less (P<0.05).In the negative control group, the number of TUNEL positive cells increased. While in the positive control and picrosideⅡgroups, the nervous behavioral malfunction, the focus infarction, the expression and concentration of iNOS and the the number of TUNEL positive cells were significantly lower than those in the negative control group (P<0.05). However, the expression and concentration of SOD were extremely higher. There was no significant difference between the positive control group and PicrosideⅡgroup (P>0.05). Conclusion PicrosideⅡmight reduce the expression of iNOS and enhance the expression of SOD to inhibit the neuronal apoptosis, so that to play a neuroprotective role in cerebral artery occlusion and reperfusion injury in rats. |
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