Expression And Correlation Of Urokinase-type Plasminogen Activator (uPA) And Its Receptor (uPAR) And Mitogen Activated Protein Kinase (MAPK) P38 In Endometrial Carcinomas

Objective:To investigate the expression of urokinase-type plasminogen activator (uPA) and its receptor(uPAR), mitogen activated protein kinase (MAPK) p38 in endometrial carcinomas, and to analyaze their correlation by examing their positive rates in the endometrial carcinomas and to investigate the relations of uPA,uPAR,P38 and the the process of invasion and metastasis of endometrial carcinomas combining the clinicopathological features (surgical pathological stage, histological grade, ermyometrial invasion and lymph metastasis). Methods:Three groups are all from the specimens of the endometrial carcinomas patients who accept surgical in the recent two years.①Group of normal endometrium:12 cases;②Group of atypical: hyperplasia20 cases;③Group of endometrial cancer specimens:50 cases.We used PV-6000 immunohistochemical method to examine the expression levels of uPA,uPAR,P38. We examined their correlations and their relations with the clinicopathological stage, histological grade and histological type of the endometrial carcinomas by examing the positive rates of the uPA,uPAR,P38 and investigate their relationships each other for the process of ivasion and metastasis of endometrial carcinomas. Results:1. The positive rates of uPA in the endometrial carcinomas patients were 58%,56%,54%, The positive rates of uPAR in the endometrial hyperplasia were 20%,25%,25%, The positive rates of P38 in the normal endometrial patients were 8.3%,16.7%,0%. The positive rates of the uPA,uPAR,P38 in endometrial carcinomas patients were highest in the three groups. The level of uPA,uPAR,P38 in the endometrial carcinomas patients were significantly higher than that in the endometrial hyperplasia and normal endometrial patients(P<0.05). The levels of uPA,uPAR,P38 in the endometrial hyperplasia patients were not significantly higher than those in the normal group (P>0.05).2. The relationship between the positive rates of uPA, uPAR and p38 and the clinicopathological features were:①Surgical pathological stage:Ⅲ-Ⅳwere higher thanⅠ-Ⅱ(x 2:4.258,8.179,5.832);②Histological grade:the lowest histological grade were higher than the higher histological grade (x 2:5.109.9.399.6.629);③Ermyometrial invasion:the deeper, the higher expression (x2:5.221,9.475.7.034);④Lymph metastasis:the expression of the uPA. uPAR,P38 were higher than none lymph metastasis (x 2: 9.805.10.464.11.15) (P<0.05);⑤Their was no relationship with the positive rates of uPA. uPAR. P38 and the pathological type(P>0.05).3. The high expression of the uPAR and the high experssion of P38 were found in the high expression of uPA in the endometrial carcinomas group, The positive cases of uPAR. P38 were significantly positively correlated with uPA positive cases(r=0.389,0.353, P<0.05). Conclusions: The interaction between uPA and uPAR plays a coordination role in the invasion and metastasis in the development of endometrial cancer, p38 may regulates uPA in the process of invasion and metastasis, so uPA, uPAR and p38 may be predict the prognosis of endometrial cancer as the important index.