Effects Of IFN-β In Vivo And Angiostatin In Vitro On Lymphangiogenesis, Respectively

Objective:This research was designed to study the effects of IFN-βin vivo and angiostatin in vitro on lymphangiogenesis, respectively.Method:1. Inhibitory experiment of angiostatin in vivo:Recovery of the cut lymphatic of the rabbit was observed.2. Culture of lymphatic endothelial cells (LE cells) of the pig thoracic duct and morphological observation:LE cells in vitro were observed by LM, confocal microscope and TEM.3. Inhibitive assays of the lymphatic endothelial cells: Method of scraping line and MTT were used to determine the inhibitory effect of IFN-βon lymphangiogenesis.4. Apoptotic assay:Hoechst staining was applied to analyze cell apoptosis.Results:1. After the lymphatic of the forefoot or hind limb of the rabbit was cut for 5 days, the lymphatic which was injected with 0.9% NaCl solution healed (control) and the one with angiostatin solution did not heal (serious leak).2. Morphological observation of LE cells was finished.3. Method of scraping line and MTT showed that IFN-βcould evidently inhibit the proliferation and migration of LE cells in vitro.4. IFN-βcould induce apoptosis of LE cells.Conclusion:1. Angiostatin has the inhibitive effect on healing of the lymphatic in vivo.2. IFN-βcould evidently inhibit the proliferation and migration of LE cells in vitro and was dose-dependent.3. IFN-βcould induce apoptosis of LE cells.