Effect Of FGL And IKVAV Petide On Spinal Cord Injury Recover In Vivo And Vivtro

Objective:Effect of FGL and IKVAV peptide on spinal cord injury recover in vivo and vivtro.Methods:(?)Cultured tne neural ce(?) mo(?)e. P(?) cells.THe P(?) cells. were divided into control and experimental groups. the experimental group added FGL peptide solution. Control group was pre-coated with poly-lysine plates. Cell proliferation was detected 1,3,5,7,9 d respectively by CCK-8②The PC-12 cells was divided into three groups, Damage control group added in H2O2 and irritated it for 16 hours. Take cells without stimulation as the normal control.The experimental group added in FGL peptide and H2O2 to irritate it for 16 hours.Apoptosis were detected by flow cytometry; The expression level of gene NF-κB was detected by fluorescence quantitative PCR.2. We synthesized IKVAV Peptides①cultured astroyte cells and the cells were divided into control and experimental groups. The experimental group added IKVAV synthetic peptides and the control group was added nothing.Three days later.The two groups were added the calcein-am/pi to detect the cell survival.②The cell were divided into normal group, control group, experimental group.Normal group was untreated.Control group did scratch.The experimental group was added IKVAV and made the scratch model.The GDNF mRNA level in astroyte was determined by real-time fluorescent quantitativePCR three days after.3.Forty-five Wistar rats were randomly divided 3 equal group:A group was sham operation group,B group undergoing SCI and injection of control peptide,C group undergoing SCI and injection of FGL peptide 30min after the operation. Paralyzed hind limb motor function were assessed with BBB score in rats.Reasult:Experimental group were higher number than the control group in the cell proliferation experiments. NF-κB mRNA expression and the number of PC-12 apoptosis in the experimental group were lower than the control group 2 Both the experimental group and control group were more than 95% survival rate. In the scratch experiments, GDNF expression in the experimental group was significantly higher than the control cells 3 In situ hybridization and Western blotting showed that expression of the CSPGS NG2 was very weak in the group A,were significently increased in the group B,and were significantly down-regulated in the group C compared with those of the group B.Conclusion:FGL peptide could promote the pc-12 cell proliferation and inhibit the apoptosis of the PC-12 cells.2 Synthetic peptide did not affect activity of cells IKVAV.The scratch injury on astoryte directly upregulated GDNFmRNA expression,and synthetic IKVAV can promote the response.3 The FGL peptide can promote the recovery of the locomotion function of the paralyzed hindlimbs by inhibinting the regulating of CSPGs NG2.