The Preparation Of N,N'-Dicarboxymethyl Chitosan-Iron Complex And Experimental Studies Of Cure Iron-deficiency Anemia

Iron is a necessary trace element for human and animals, it plays an important role in metabolic processes, iron deficiency can lead to iron-deficiency anemia. Treatment of iron deficiency anemia drug ingredients can be divided into inorganic iron and organic iron, according to its existence in the body, can be divided into heme iron and nonheme iron. Among them, ferro ion can absorb much better than ferri ion for human, organic iron is better than inorganic iron, and the irritation in stomach and intestine which cause by organic iron is smaller than inorganic iron. In recent years, various use polysaccharide iron complexs such as organic iron to treat anemia, experimental studys are very hot.Chitosan is a production of chitin disacetyl,it is a natural polysaccharides material, it has the biocompatibility, biodegradation and low toxicity, almost no allergies characteristics.Therefore, in biomedical fields has wide application prospects. Because of the water-solubility of chitosan was not good, this greatly restricted its application scope. After chemical modification of carboxymethyl chitosan its water-solubility greatly improved, ion complexation and performance increase consequently.This experimental design developed a new type of polysaccharide iron supplement, and is applied to the treatment for anemia mice. To built an experimental basis for developping relevant iron agent. This paper includes the following sections:1. Through the chemical modification of chitosan, first prepare the water-soluble N,N′-dicarboxymethyl chitosan, based on this, to study the capability of N,N′-dicarboxymethyl chitosan(DCMC) complexing with Fe2+ and Fe3+, preparation of N,N', that the iron carboxymethyl chitosan optimal experiment condition: pH=5.6, the Fe2+ concentration of 0.04 mol/L, for 20 min at room temperature, reaction, N,N′-dicarboxymethyl chitosan-Fe(Ⅱ) on the adsorption quantity biggest for 0.421 g/g; pH=7, the Fe3+ concentration of 0.04 mol/L, for 20 min at room temperature, reaction, N,N′-dicarboxymethyl chitosan-Fe(Ⅲ) on the adsorption quantity biggest for 0.397 g/g. DCMC-Fe(Ⅱ) and DCMC-Fe(Ⅲ) two complexes in pH 1~10 are stable, DCMC and iron ions adsorption is a stable complexes form, no free iron ion exist.2. Will N,N′-dicarboxymethyl chitosan-iron complex acute toxicity experiment research, refer to state acute toxicity (LD50) dose grading standards, N,N′-dicarboxymethyl chitosan-iron complex without toxic, clinical application of safety and reliability. To ferrous sulfate as reference substance, to N,N′-dicarboxymethyl chitosan-iron complex gastric mucosal excitant research, draw the conclusion N,N′-dicarboxymethyl chitosan-iron complex the gastric mucosa is less than that of ferrous sulfate irritating.3. To ferrous sulfate as reference substance, using the N,N′-dicarboxymethyl chitosan-iron complex to anemia treated mice, experimental results show that N,N′-dicarboxymethyl chitosan-iron complex supplement iron superior to ferrous sulfate, effect of the relative bioavailability of ferrous sulfate 121% can be achieved.4. Based on the basic characteristics of DCMC-Fe(Ⅱ), preliminary design DCMC-Fe(Ⅱ) primarily medicine (stomach soluble granule-coating piece type). Determine the core preparation methods for dry powder tablet tablets of orthogonal design method, and the optimum piece experiment of the core prescription. In effect, the release drugs coating the integrity of the coating film degree and investigated for target, the influence factors of coating process, thus get the best technological conditions.