Effect Of Yuzhi Capsule On Alcoholic Fatty Liver And Its Mechanisms In Rats

Along with the change of people's life style and the improvement of living level,alcohol consumption also increases year after year, resulting in alcoholic liver injury also anincreasing trend. Alcohol has become the second largest cause of hepatic injury after theviral liver disease. Alcoholic fatty liver, an important stage of alcoholic liver injury, hasbecome a serious public health problem, and has terribly harm to human health. Nowalcoholic fatty liver has become a hotspot of medical and digestive research.At present, domestic and foreign researchers study the pathogenesis and therapeuticeffect of drugs mainly through the establishment of animal model of alcoholic fatty liverdisease. The Lieber-Decarli model is a successful one. Rats are fed a special liquidingredients, containing liquid food nutrients and a certain concentration of alcohol, whichcan guarantee the rat adequate intake of food nutrition and enough alcohol for modeling.4weeks later the model rats appears apparent fatty lesions. Based on Lieber-Decarli model,Tsukamoto-French uses the operation way to implant a gastric tube in rat. The fluidingredients containing alcohol are continuously injected into the rat stomach.Theconcentration of alcolhol can be upto47%in this model. The main advantage ofTsukamoto-French model is that the amount of alcohol can be set to any value. Rats of themodel group emerge not only of fatty liver,but also inflammatory infiltration and necrosis ofliver cells. Along with the research needs, researchers add auxiliary agent in the liquidingredients, such as endotoxin and iron to establish the alcoholic hepatic injury model. Theseauxiliary agents could inhibit the alcohol metabolism, directly injure the liver cells andinduce liver lipid peroxidation. The domestic scholars mainly give rats a amount of alcoholto establish the model of alcoholic fatty liver through mandatory. This method eliminates theneed for surgical operation, and the method is simple, with advantages of low cost, stabilityand reproducibility. This method obtain a widespread application. Our experiments usingthis method to reproduce the model of alcoholic fatty liver successfully.The pathogenesis mechanisms of alcoholic fatty liver have multiple aspects, which havebecome a hotspot of research scholars at home and abroad, mainly related to multipledisciplines such as physiological, biochemical, immunological, genetic and endocrine. Thetoxicity of alcohol and its metabolite acetaldehyde in the liver is the most direct cause. Alcohol can inhibit the activity of alcohol dehydrogenase system, making the NAD+shiftfor NADH and the mitochondrial NADH increase. This may inhibit the three tricarboxylicacid cycle and fatty acid beta oxidation, resulting in fatty acid accumulation. It also canmake the fatty acid synthase high activity, ultimately promoting fatty liver. The ethanolmetabolite acetaldehyde has very strong liver toxicity, also can damage mitochondria andthree tricarboxylic acid cycle, resulting in a large number of free radicals and lipidperoxidation. Oxidative stress and lipid peroxidation is another important reason in alcoholicfatty liver formation. Oxidative stress, resulting in a large number of oxidative stressproducts such as superoxide anion free radical, hydroxyl radical and H2O2, can degradatemembrane phospholipids, which change the membrane-bound enzymes, receptors and ionchannels in the microenvironment, and affect the function or the formation of new ionchannel. MDA, the product of lipid peroxidation, may make adducts with proteins, activatingimmune response in liver injury. In addition to the above factors, factors like endorphin, iron,inflammatory mediators and cytokines and endotoxin can cause liver cell damage through avariety of mechanisms, ultimately leading to the formation of fatty liver.The Yu Zhi capsule in our experiments consists mainly of turmeric, PSK, bupleurumand sarmentosum and other effective components, uniformly mixed in a certain proportion,filled into capsule. Turmeric mainly containing volatile oil, which has camphene andcamphor and other active ingredients, can reduce serum lipid effect. Coriolus VersicolorPolysaccharide, extract of coriolus versicolor is the main active ingredient. CoriolusVersicolor Polysaccharide with liver protecting effect, can significantly reduce the serumtransaminase, and have distinct repair effect liver tissue lesions and hepatic necrosis.Bupleurum main composition containing saikosaponin, sterols, main components, canenhance immunity and anti liver injury in mice. N-methyl isopelletierine main chemical ofsedum sarmentosum compositions has a strong protective effect of liver. A mixture of theabove effective ingredients can play a very strong protection of hepatic cells, promoting theregeneration of liver cells, fat, resisting free radical, anti-inflammatory, inhibition of hepaticcell apoptosis. And it can play a certain role in prevention and treatment of alcoholic fattyliver.To sum up, our study based on the combination of pathogenesis of alcoholic fatty liverdisease and pharmacology of traditional Chinese medicine, develop of a new preparation ofcompound--Yu Zhi capsule, mixed of turmeric, PSK, bupleurum and sarmentosum andother effective components. The model of alcoholic fatty liver were successfully established using alcohol by gavage method. Yu Zhi capsules were given to the rats of model. The resultrevealed that Yu Zhi capsule has a very good treatment and protection on alcoholic fatty liver,and presents a certain dose-dependent. Its possible mechanism is that it can reduce the livertissue and serum lipid, increase antioxidant activity, and remove the vivo peroxidationproduct. Our study provide an important research direction, and a theoretical basis forclinical application of Yuzhi capsule.