| 1. Extract of Bulbus Allium Fistulosumon Lipid Peroxidationof Non-alcoholic Fatty Liver Disease in Clinical OberservationPurpose:observation extract of bulbus allium fistulosum in patients with NAFLD clinical efficacy, related biochemical indicators of lipid oxidation indicators, security, research the scallion stalk extract prevention of NAFLD mechanism, and focus research Onion extract an important part of the pathogenesis of NAFLD lipid peroxidation in rats, and to provide a scientific basis for the continued development and research.Method:In November2011January2013cases in the Wuhan hospital outpatient and digestive medicine inpatient ward for treatment, consistent with nonalcoholic fatty liver Tanning the blood stasis diagnostic standards cases were randomly divided into78cases39people in each treatment group and the control group. The treatment group received oral extract of bulbus allium fistulosum, the control group was treated Essentiale soft capsules, three months for a course of treatment effects were observed after2weeks off. Observed two groups of patients, clinical efficacy, TCM syndrome, body mass index (BMI), B super results, serum triglyceride (TG), total cholesterol (TC), high density lipoprotein (HDL), low-density lipoprotein (LDL), alanine aminotransferase (ALT), aspartate aminotransferase the enzymatic (AST), serum superoxide dismutase (SOD), malondialdehyde (MDA), the changes were statistically analyzed.Results:the results of clinical effects of the treatment group total effective rate was87.18%,69.23%better than the control group; TCM syndromes results of treatment group, the total effective rate was89.74%,71.80%better than the control group; treatment group and the control group than each treatment before the BMI results change significantly improved (P<0.01), but the two groups after treatment comparison the BMI was not statistically significant (P>0.05); treatment group and the control group than their respective pre-treatment ALT, AST, TG, TC, LDL decreased significantly (P<0.01) HDL level was no significant difference (P>0.05), but the two groups after treatment, ALT, AST, TG, TC, and HDL, LDL level was not statistically difference (P>0.05); treatment group and the control group Essentiale than each treatment before MDA decreased SOD increased significantly (P <0.01), but the two groups after treatment comparison, SOD, MDA was no statistically significant difference (P>0.05).Conclusion:extract of bulbus allium fistulosum patients of non-alcoholic fatty liver Tanning clinical symptoms, body mass index, liver function, blood lipids, lipid peroxidation indicators, the results of the B-mode images have good improvement and therapeutic effect. extract of bulbus allium fistulosum on liver lipid deposition, oxidative stress and lipid peroxidation links, reduced liver lipid deposition, improve oxygen stress and lipid peroxidation, corrected resulting in mitochondrial damage, liver the steatosis ultimately played a role in the prevention and control of non-alcoholic fatty liver disease.2. Extract of Bulbus Allium FistulosumInduced PGC-1α on on Lipid Peroxidationof Non-alcoholic Fatty Liver Disease in Experimental ReseachObjective:To observe after extract of bulbus allium fistulosum interventions and the inhibition of PGC-1a and excited intervention, non-alcoholic fatty liver disease rat model of change, and hepatic lipid peroxidation level changes discussed extract of bulbus allium fistulosum of non-alcoholic fatty liver the preventive and therapeutic effects, and the effects of lipid peroxidation.Methods:99Wistar rats were randomly divided into two groups, the normal group of18, high fat diet for81model to establish a rat model of nonalcoholic fatty liver disease, high fat diet through improved modeling after random choose the the nine normal group and nine high fat diet model groups were sacrificed to confirm successful modeling. The remaining72high fat diet model groups and nine normal rats were randomly divided into9groups (n=9): normal group, model group, extract of bulbus allium fistulosumgroup, polyene phosphatidyl choline group, extract of bulbus allium fistulosum+Forskolin agonist group, light blue extract+H-89inhibitor group, forskolin agonist Group, H-89inhibition hardener group, dimethyl sulfoxide group. Each group were given the Onion extract drug intragastric and intraperitoneal injection therapy. Drug intervention4wk,9rats were sacrificed, calculated liver index (LI), HE staining, Sudan IV staining electron microscopy for histopathological examination, biochemical serum triglyceride (TG), total cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDL), alanine aminotransferase (ALT), aspartate amino transferase (AST) content, colorimetric determination of liver tissue MDA, SOD content.Results:The analysis of non-alcoholic fatty liver rat model of liver index, serum ALT, AST, TG, TC, LDL indicators, found Onion extract+Forskolin agonist group above indicators to improve the effect of the best Onion extract group, and more polyene phosphatidylcholine group, Onion extract+H-89inhibitor group, Forskolin the agonist group these indicators have varying degrees of improvement, while the H-89inhibitor group have these indicators severity. Analysis of non-alcoholic fatty liver rat model of lipid over the oxidation indexes MDA, SOD Onion extract, polyene phosphatidyl choline groups, extract of bulbus allium fistulosum+H-89inhibitor group, extract of bulbus allium fistulosum+Forskolin agonist group, H-89inhibitor group have different degrees of lowering effect on the MDA, have different degrees of elevated role of SOD, MDA increased Forskolin agonist group, SOD reduced. The analysis of the experimental results found in this experiment, all indicators of dimethyl sulfoxide, with the model group had no significant difference in the dimethyl sulfoxide as a good solvent, has no effect on the experimental results.Discussion:Onion extract by inducing the expression of PGC-1α increased significantly improve the fatty acid β-oxidation, reduce excessive free fatty acid accumulation in the liver, and enhance the liver triglycerides output, lower serum TG, TC, reduce liver lipid The extent of deposition, at the same time to reduce the content of MDA, increased SOD content to play a role in the prevention and control of non-alcoholic fatty liver disease. On the other hand, an increase in the expression of PGC-1α cause lipid peroxidation heavier. Onion extract may play a role in the prevention and control of non-alcoholic fatty liver disease through induction of PGC-1a in lipid peroxidation point, specific regulation and mechanism of action remains to be further more in-depth research and exploration. |
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