The Biological Performance Of BMMSCs And Their Interaction With Spinal Cord Repair Materials

In this paper, the preliminary cytology evaluation of Spinal Cord Injury(SCI) repair scaffold materials(grafted IKVAV poly lactic acid polyethylene glycol hydrogel) was made based on the cell foundation of rat bone marrow mesenchymal stem cells(BMMSCs). Cell proliferation, cell cycle, cell toxicity and cell adhesion assays were done through the co-culture of cell and materials of to explore the cells compatibility of SCI repair scaffold materials. The main research results are as follows:The full bone marrow adherence method was employed to isolate and culture rat BMMSCs, the cells were purified by frequently medium change and the control of initial detachment time. Cells had a more unified morphology from the third generation which meaned cell purity was improved. The biology performance of BMMSCs was discussed via cell growth curve drawing, the cell cycle determination, cloning unite formation experiments and induction into bone and fat experiment. The experimental results showed that our cells is high purity and has unified morphology, exuberant proliferation ability and a multiplex differentiation potential, they are BMMSCs.BMMSCs were inoculated in24h in the plate which were coated by five different density IKVAV peptide solution, the adherence rate test results show that the concentration of0.1mM peptides solution is the most suitable for the cell to adhere. The cell proliferation results influence by five peptides solution showed that: The OD value of0.1mM peptide group was greater than other groups, the promote role on cell proliferation is obvious, significant effects on the cell vitality. Flow cytometric cycle tests of BMMSCs under the action of the peptide solution showed that:32.29%of0.1mM group of cells was in S phase, the proliferation situation is better than other groups. The experimental results above show that: IKVAV solution can promote cells to adhere and grow faster in different degree.The microscope pictures of3D culture of materials and BMMSCs showed that the cells in the grafted IKVAV hydrogel growed evenly, had a long spindle shape. Cells were sparse in the non-grafting hydrogel. SEM figures showed that the cells can be a very good adhesion to the surface of IKVAV grafted hydrogel, and almost no cell adhesion on non-grafting hydrogel.