Clinical Study Of PECAM-1Expression In Blood Cells Of DVT Aggravating Inflammation And Promoting Thrombosis

[Objective]On the basis of gene chip screening for deep venous thrombosis(DVT) in rat endothelial cells differentially expressed genes in the early days, access to the latest relevant literature found in platelet endothelial cell adhesion molecule-1(PECAM-1/CD31)in leukocytes, platelets,endothelial cellsand other organizations have expressed, this experiment at leukocytes and platelets, focus on deep venous thrombosis in acute period (≤7d), through the PCR detection of PECAM-1mRNA expression, analysis of its possible involvement in acute DVT.[Materials and methods]1.It is collected161cases from2010.3to2012.3and in accordance with Venous thromboembolism prevention guide for NICE and Diagnosis of DVT Antithrombotic Therapy and Prevention of Thrombosis:American College of Chest Physicians Evidence-Based Clinical Practice Guidelines (9th Ed) developed in the present study the diagnostic criteria of DVT:DVT=comprehensive diagnosis risk factors assessment (risk factors based+specialist hazardous factors)+the main clinical manifestations+limb deep venous by color Doppler ultrasonography (Appendix1). Finally in this study58cases and according to three groups into the exclusion standard into normal control group (n=20, healthy volunteers), non-thrombosis group (n=20, for long bone fracture of lower limb or spine fracture7d experts after the operation without thrombosis patients), thrombosis group (n=18, patients forming DVT), age, sex, body mass index of three groups have no statistically significant differences. non-thrombosis group and thrombosis group according to" Venous thromboembolism prevention guide for NICE" standard processing. The three groups were collected fasting venous blood samples, non-thrombosis group in operation after1d acquisition, thrombosis group diagnosed after1d acquisition, in strict accordance with the collection point blood specimen collection time after processing save spare.2. Routine blood test and by GAPDH as the internal reference,PECAM-lmRNA of leukocytes in blood samples is detected by PCR, observing the expression changes in acute DVT.[Result]Patients with acute DVT white blood cells of the quantity, neutrophils ratio, CRP testing higher than conventional normal control group, thrombosis, failure to form groups; PCR detection results show that:PECAM-1mRNA in thrombosis group a normal control group, blood clots forming groups are not express increases, exist differences (P<0.05).[Conclusion]Patients with acute DVT exist the aggravation of inflammation phenomenon, verify the related reports in the literature of thrombosis increase inflammatory response in the conclusion; At the same time, PECAM-1mRNA level increased expression, probably with its increase inflammation, promote thrombosis relevant.