| Objective:rats after myocardial infarction ventricular remodeling mode,to study the effect of recombinant human erythropoietin(rHu-EPO) at different time periods on hemodynamic,ventricular function and infarct size of left ventricle in rats with myocardial infarction.Methods:60healthy male Sprague Dawley rats were divided randomly and equally into5group:sham group, simple cardiac remodeling after myocardial infarction group, the intervention group of different drugs (rHu-EPO in the intervention group and SB203580group, rHu-EPO+SB203580,group). Dose of30mg/kg of intraperitoneal injection of3%sodium pentobarbital anesthesia. Middle incision thoracotomy, small animals distraction distraction thoracic tracheal intubation to connect small-animal ventilator. Along the anterior interventricular groove to distinguish Traveling anterior descending coronary artery, left anterior descending branch of the upper1/3at threading ligation, the blood supply to the myocardium purple and multi-channel physiological recorder showed ST-segment elevation blocking the hallmarks of success. Heart back off the chest for4weeks. Different drugs in the intervention group before ischemia began subcutaneous injection of drugs, later twice a week. The rHu the EPO dose for1500U/kg. SB203580dose of0.2mg/kg. Respectively, after24hours,2weeks,4weeks Determination of hemodynamic parameters, recording of left ventricular systolic pressure (LVSP), left ventricular end diastolic pressure (LVEDP), maximal rate of left ventricular pressure (+dp/dt) and left ventricular pressure decline rate (-dp/dt), and record the synchronization of heart rate (HR). The animals were sacrificed4weeks after the collection of heart specimens, according to the left and rightventricular weight (LVW, RVW), the relative weight calculation of left and right ventricular (LV/BW in the RV/BW). TTC and Evans blue staining was used to detect left ventricular infarct size, and pathological examination of gross and microscopic morphological change.Results:24hours after operation:Compared with the sham group, simply myocardial infarction cardiac remodeling group left ventricular systolic pressure (LVSP), left ventricular end diastolic pressure (LVEDP) and left ventricular pressure maximum rise and fall rate (±dp/dt) was significantly abnormal, LVSP and±dp/dt were significantly reduced, the LVEDP was significantly increased (P <0.05); compared with after simply myocardial infarction cardiac remodeling group, the intervention group (rHu-EPO in the intervention group, SB203580group, rHu-the EPO+SB203580group)±the dp/dt improved significantly (P <0.05). After2weeks: compared with the sham group, a simple cardiac remodeling after myocardial infarction group LVSP and LVEDP and±dp/dt significant deterioration (P﹤O.05); compared with after simply myocardial infarction cardiac remodeling group, different drugs The intervention group (rHu-EPO in the intervention group, SB203580group, rHu-the EPO+SB203580group)±the dp/dt was significantly improved (P <0.05). After4weeks:compared with the sham group, a simple cardiac remodeling after myocardial infarction group LVSP and LVEDP and±dp/dt significant deterioration (P <0.05); compared with after simply myocardial infarction cardiac remodeling group, different drugs The intervention group (rHu-EPO in the intervention group, SB203580group, rHu-the EPO+SB203580group)±the dp/dt was significantly improved (P <0.05). Compared with the sham group, simply myocardial infarction cardiac remodeling groups the LV/BW increased, the difference was statistically significant (P <0.05). Compared with after simply myocardial infarction cardiac remodeling group, the intervention group (rHu-EPO in the intervention group and SB203580group, rHu-the EPO+SB203580group) LV/BW decreased, the difference was statistically significant (P<0.05). Compared with after simply myocardial infarction cardiac remodeling group, the intervention group (rHu-EPO in the intervention group and SB203580group, rHu-EPO+SB203580group) cardiac infarct size was significantly reduced (P <0.05). HE staining and Picric acid Sirius red staining Microscopy after found compared with after simply myocardial infarction cardiac remodeling group, different drugs The intervention group (rHu-EPO in the intervention group, SB203580group, rHu-the EPO+SB203580group) Inflammatory cell infiltration and Cardiac myocyte fibrosis significantly reduced,A lot of capillary regeneration In Myocardial infarction border area.Conclusion:RHu-EPO can significantly improve myocardial infarction rats after cardiac function, increase LVSP,±dp/dt, reduce LVEDP. Reduce LV/BW, through the narrow myocardial infarction rats heart infarction area, reduce infarction border area inflammatory cells infiltrating and cardiac muscle cell fibrosis, promote blood capillary freshman, inhibit ventricular remodeling. |
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