| Objective:To study the protective effect of Panax Notoginseng Saponin in the hypoxic-ischemic brain injury through establishing the classic neonatal rat model with neonatal SD rats atmospheric asphyxia model. By testing the expressions of HO-1protein and Bax protein in brain tissues, study the effect of PNS in them and the protective effect of Panax Notoginseng Saponin in hypoxic-ischemic brain injury. And study the effective time of the treatment of neonatal brain damage. To seek new theoretical foundations for the treatment of hypoxic-ischemic brain injury after neonatal asphyxia.Methods:887-10day-old newborn SD rats were randomly divided into the control group(n=8,group I),saline-treated group(n=40,group II), and Panax Notoginseng Saponin-treated group(n=40, group III). Right after hypoxic-ischemic insult, PNS and normal saline solution were injected intraperitoneally. Normal Saline-treated group and PNS-treated group were also randomly divided into five groups for6hours(group6h),24hours(group24h),48hours(group48h),72hours(group72h),7days(group7d). Every group had8newborn SD rats. The newborn rats of PNS-treated group were given100mg/kg PNS by intraperitoneal injection as soon as the neonatal rat model with neonatal rat atmospheric Asphyxia model were established, the same dose of PNS was repeated by intraperitoneal injection every12hours. The newborn rats of Normal Saline-treated group were given the same volume of normal saline as PNS by intraperitoneal injection.At the end of the study, group I of neonatal rats were broken ends and taken the brains together, group II and III were given the same treatment respectively in6hours,24hours,48hours,72hours,7days after asphyxia, and then the brain tissues were fixed, paraffin-embedded and made into paraffin sections. And took the steps of HE staining and immunohistochemical,the latter could help us to detect the expressions of HO-1and Bax protein. Brain tissues were also harvested for measurement using HE. At last the data were processed with SPSS17.0statistical method, including chi-square tests and paired T tests.Results:(1) By chi-square tests were conducted within group comparisons, we summarized that①in the Saline-treated group, the expression of HO-1protein at each time point had significant statistical difference (p<0.01);②in the PNS-treated group, the expression of HO-1protein at each time point had significant statistical difference (p<0.01);③in the Saline-treated group, the expression of Bax protein at each time point had statistical difference (p<0.05);④in the PNS-treated group, the expression of Bax protein at each time point had statistics difference (p<0.005).(2)By paired T test which was used between groups, we concluded that①Saline-treated group and PNS-treated group's expression of HO-1was significantly higher than the control group (p<0.01), and reached the peak in24hours;②PNS-treated group's expression of HO-1was significantly higher than saline-treated group(p<0.05);③saline-treated group and PNS-treated group's expression of Bax was significantly higher than the control group (p<0.01), and reached the peak in48hours;④PNS-treated group's expression of Bax was significantly lower than saline-treated group (p<0.05).Conclusion:①HO-1was one of protecting proteins.which hardly expressed in the normal brain tissues. The expression of HO-1increased after neonatal SD rats asphyxia, which showed that HO-1had protective effect in hypoxic-ischemic brain damage;②Bax was one of apoptosis proteins, and seldom expressed in normal tissues. Its expression increased after neonatal SD rats asphyxia, which showed that Bax protein could increase apoptosis of cells in hypoxic-ischemic brain damage;③The use of PNS could increase the expression of HO-1protein, and reduced the expression of Bax protein of brain tissues in hypoxic-ischemic brain damage, at last protected the brain tissues, which indicated that PNS had significant protective effect in brain damage caused by asphyxia. |
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