Effect Of HPV And HP On The Incidence Of Esophageal Cancer And Its Association With P53and P63

Objective: To investigate the effect of human papilloma virus (HPV) andHelicobacter pylori (H. Pylori) on the incidence of esophageal cancer and theunderlying mechanisms.Methods:1Esophageal cancer tissue specimens were taken from the Third chestsurgery of Fourth Hospital of Hebei Medical University between September of2010to September of2012. Each specimens contain cancer tissue andparacancerous tissue, the paracancerous tissue were taken from2cm awayfrom the edge of the tumor. Normal controls esophageal mucosa tissues wereobtained from the same period healthy people of Fourth Hospital of HebeiMedical University, whose physical examination showed no other malignantdiseases and without esophageal cancer history. Esophageal cancer group andnormal control group specimens were collected25cases respectively.2DNA were respectively extracted used the DNA extraction kit which isbuy from QIAGENE company. Expression of HPV and H. Pylori in cancertissue, paracancerous tissue and normal controls esophageal mucosa tissuewere detected by PCR, and analysis the effect of human papilloma virus (HPV)and Helicobacter pylori (H. Pylori) on the incidence of esophageal cancer.3Expression of p53in cancer tissue and normal esophageal tissue weredetected by immunohistochemical staining technique, and analysis the effectof p53on the incidence of esophageal cancer, and analysis the relevancebetween H. Pylori and p53. To study H. Pylori whether through p53proteinexpression influence the incidence of esophageal cancer.4Expression of p63in cancer tissue and normal esophageal tissue weredetected by PCR, and analysis the effect of p63on the incidence of esophagealcancer, and analysis the relevance between H. Pylori and p63. To study H. Pylori whether through p63protein expression influence the incidence ofesophageal cancer.Results:1The infection of Helicobacter pylori in esophageal cancer: PCRanalysis showed that the positive expression rate of Helicobacter pylori inesophageal tissue was68%, that were higher compared with the normaltissue. The result had statistical meaning (P<0.05). That suggested that theHelicobacter pylori infection maybe a reason of esophageal cancer.2The infection of human papillomavirus in esophageal cancer: ThePCR results showed that the tissues taken from patients with esophagealcancer, paraneoplastic and normal esophageal tissues, the expression of thehuman papillomavirus (HPV) were negative. That suggested the effect ofhuman papillomavirus was not a reason of esophageal cancer.3Cross-infection in patients with esophageal cancer humanpapillomavirus and Helicobacter pylori: Analyzing of result1and2, thepositive rate of Helicobacter pylori in patients with esophageal cancer was68%, while that of human papillomavirus infection is0%, suggesting thatthere was no cross-infection between human papillomavirus and Helicobacterpylori in patients with esophageal cancer.4The expression of mutant P53protein in esophageal carcinoma: Thepositive rate of mutant p53protein expression in esophageal carcinoma was52%and that was obviously higher than in normal esophageal tissues (4%).The result had statistical meaning (P<0.05). That suggested there hadrelationship between esophageal cancer tissue and mutant p53protein, and theexpression of p53was correlated with the degree of cell differentiation andwithout lymph node metastasis.5The relevance of esophageal cancer patients with Helicobacter pyloriinfection with mutant p53expression: Helicobacter pylori infection in patientswith esophageal cancer was positively correlated with the expression ofmutant p53protein, suggesting that Hp infection relate with the increasingexpression of mutant p53protein in the progression of esophageal cancer. 6Expression of p63DNA in esophageal carcinoma: The PCR analysisshowed that, there was significant difference of the positive rate of ΔNp63gene between esophageal cancer tissues and normal tissues (P<0.05). Theexpression of ΔNp63gene was related with pathologic staging of esophagealcancer and without lymph node metastasis. The TAp63gene expression ofesophageal cancer tissue and normal esophageal mucosa were negative,indicating that the process of esophageal cancer with ΔNp63and TAp63hasnothing to do.7The relationship of ΔNp63with Helicobacter pylori in patients withesophageal cancer: The infection of Helicobacter pylori and the expression ofΔNp63gene was not related, suggesting there did not had relationshipbetween the expression of ΔNp63gene and the infection of Helicobacterpylori in the progression of esophageal cancer.Conclusion:1There was significant difference between the esophageal tissue andnormal tissue of the infection of Helicobacter pylori, suggesting the infectionof Helicobacter pylori maybe a reason of esophageal cancer.2The positive rate of Helicobacter pylori in esophageal cancer was68%and that of human papillomavirus (HPV) was0%, suggesting there did notexist cross-infection between Helicobacter pylori and HPV in esophagealcancer.3The process of esophageal cancer was related with the expression ofmutant p53and ΔNp63gene, suggesting the two gene play a key role in theformation of esophageal cancer.4The infection of Helicobacter pylori was positive correlated with theexpression of mutant p53gene in the esophageal cancer process and withoutthe expression ofΔNp63gene.