The Correlation Study Of Pathogenesis Between CD147/Basigin,TAP63/△ NP63 And Eyelid Basal Cell Carcinoma

Objectives: To investigate the expression of CD147/basagin in eyelid basal cell carcinoma(BCC) and the correlation between its expression and prognosis. To study the expression of MMP2 in eyelid BCC, analyze its relationship with CD147. To study the expression of ΔNP63/ TAP63 in nomal skin and eyelid BCC , in order to reveal its molecule mechanism in tumorigenesis and epidermis alternation.Methods: The expression of CD147 was detected in eyelid BCC of 25 patients by fluorescent quantitation PCR and laser scanning confocal microscope immunofluorescence, the correlation between its expression and tumour recurrence,histopathology grouping were also studied. In order to analyze the relationship of MMP2 production in eyelid BCC tumour cell and matrix cell with the expression of CD147, the protein expression of MMP2 was detected by immunohistochemistry. The expression of A NP63/ TAP63 in eyelid BCC was detected by fluorescent quantitation PCR, and in different cell of normal skin fluorescent quantitation nest PCR was used. Results: (1)The RNA transcription of CD147 was detected in 25 eyelid BCC cases and only in 6 normal skin, and correspondence copy number of normalskin were more lower than eyelid BCC cases. The immunofluorescence confirmed this result. (2) The positive expression rate of MMP2 in differentiation group ^ primary undifferentiation group and recurrence undifferentiation group was separately: 40%> 47.06% and 100%, and the correlation between MMP2 and tumour recurrence was found. (3) Significant correlation was found between CD 147 expression and matrix cell, no correlation was found between CD 147 expression and eyelid BCC tumour cell. (4) The expression of A NP63 was higher than TAP63 in eyelid BCC and normal skin, and the correspondence copy number of A NP63 in eyelid BCC was significant higher than normal skin. (5) A NP63 was mainly expressed in basal layer cell and hair follicle stem cell, terminally differentiated cells almost negtive for ANP63. There was no statistical significance of TAP63 between basal layer celK hair follicle stem cell and matrix cell.Conclusion: The expression of MMP2 in eyelid BCC tumour cell and matrix cell were correlated with tumorous invasion and recurrence. CD 147 might stimulat expression of MMP2 in matrix cell of eyelid BCC. A NP63 may play an important role in the progression of eyelid BCC. Variability expression of A NP63 in eyelid BCC indicate different biological behaviour and clinic turnover of tumour cell ,and could be the index of prognosis.